摘要
目的 探讨津力达颗粒对高糖作用下MIN6细胞功能的影响。方法 根据不同培养条件将MIN6细胞分为空白组、100 mg/L津力达组、200 mg/L津力达组、400 mg/L津力达组,分别在磷酸盐缓冲溶液(phosphate buffered saline, PBS)、含100 mg/L津力达溶液,含200 mg/L津力达溶液,含400 mg/L津力达溶液中培养,培养48 h后,再分别加入浓度为2.8、16.7 mmol/L的葡萄糖KRBH(Krebs-Ringer bicarbonate HEPES)缓冲液中孵育0.5 h。根据不同培养条件将MIN6细胞分为NG组(5.5 mmol/L葡萄糖)、NG+津力达组(5.5 mmol/L葡萄糖+200 mg/L津力达)、HG组(25 mmol/L葡萄糖)、HG+津力达组(25 mmol/L葡萄糖+200 mg/L津力达),培养48 h。体外建立小鼠胰岛微血管内皮细胞与MIN6共培养模型,按正糖(N)、高糖(H)不同干预和加用津力达(J)分为N1+N6组、N1+J+N6组、N1+H6组、N1+J+H6组、H1+N6组、H1+J+N6组、H1+H6组、H1+J+H6组。收集各组细胞及上清测定胰岛素(insulin, INS)水平。结果 16.7 mmol/L葡萄糖刺激时,空白组、100 mg/L津力达组和200 mg/L津力达组细胞内及上清中INS水平随津力达浓度升高逐渐增高(P均<0.05)。相同浓度津力达条件下,16.7 mmol/L较2.8 mmol/L葡萄糖刺激时各组细胞内及上清INS水平均上升(P均<0.05)。HG组较NG组细胞及上清INS水平降低(P均<0.001),HG+津力达组较HG组INS水平升高(P均<0.001)。N1+H6、H1+N6、H1+H6组较N1+N6组的细胞内和上清INS水平均降低(P均<0.05)。高糖加入津力达的各共培养组细胞内及上清INS水平均较对应未加入组升高(P均<0.001)。结论 高糖刺激时,津力达在一定范围内呈浓度依赖性促进β细胞合成和分泌INS,而低糖时无此作用。持续高糖环境下津力达既可直接促进β细胞INS的合成和分泌,还能通过调节胰岛微血管内皮细胞改善β细胞功能。
Objective To explore the effects of Jinlida granule on MIN6 cell function under the effect of high glucose. Methods According to different culture condition, MIN6 cells were divided into blank group, 100 mg/L Jinlida granule group, 200 mg/L Jinlida granules group and 400 mg/L Jinlida granules group. The cells were cultured in phosphate buffered saline(PBS), 100 mg/L, 200 mg/L and 400 mg/L Jinlida solutions respectively;after 48 hours of culture, 2.8 mmol/L and 16.7 mmol/L glucose KRBH(Krebs-Ringer bicarbonate HEPES) was added to incubate for 0.5 hour. According to different culture condition, MIN6 cells were divided into NG group(5.5 mmol/L glucose), NG+ Jinlida group(5.5 mmol/L glucose +200 mg/L Jinlida), HG group(25 mmol/L glucose), HG+ Jinlida group(25 mmol/L glucose +200 mg/L Jinlida), which cultured for 48 hours. Mice islet microvascular endothelial cells were co-cultured with MIN6 in vitro and according to normal glucose(N), high glucose(H) and Jinlida(J) intervention were divided into N1+N6 group, N1+J+N6 group, N1+H6 group, N1+J+H6 group, H1+N6 group, H1+J+N6 group, H1+H6 group, H1+J+H6 group. Cells and supernatant of each group were collected to determine insulin(INS) levels. Results When stimulated by 16.7 mmol/L glucose, INS level in cells and supernatant of blank group, 100 mg/L Jinlida group and 200 mg/L Jinlida group increased gradually with increasing Jinlida concentration(all P<0.05). Under the same concentration of Jinlida, the INS levels in cells and supernatants of 16.7 mmol/L were higher than those of 2.8 mmol/L glucose stimulation(all P<0.05). The INS level of cells and supernatants in HG group was lower than that in NG group(all P<0.001), and the INS level in HG+Jinlida group was higher than that in HG group(all P<0.001). Compared with N1+N6 group, the intracellular and supernatant INS levels in N1+H6, H1+H6 groups were significantly decreased(all P<0.05). The level of INS in cells and supernatants of co-culture groups with high glucose added Jinlida was higher than that of the group without
作者
陈伟
王之旸
付友娟
刘子微
乐岭
CHEN Wei;WANG Zhiyang;FU Youjuan;LIU Ziwei;YUE Ling(Department of Endocrinology,General Hospital of Central Theater Command,Wuhan Hubei 430070,China)
出处
《华南国防医学杂志》
CAS
2022年第8期594-598,共5页
Military Medical Journal of South China
