摘要
细胞壁是一种复杂的动态网络结构,在植物生长发育、胁迫应答和免疫抗性过程中起着重要的调控和防御作用。果胶(pectin)是细胞初生壁结构中多糖的主要成分之一;其中,同型半乳糖醛酸聚糖(HG)是果胶多糖组分中含量最丰富的线性聚合物。HG的甲基酯化程度变化会导致其酶解形成凝胶,从而影响果胶结构的稳定性。果胶甲酯酶抑制蛋白(PMEIs)通过翻译后机制调控果胶甲酯酶(PMEs)活性,微调果胶多糖甲酯化修饰平衡后,维持细胞壁的完整性和生物力学特性。研究发现,PMEI-PME互作调控果胶甲酯化修饰的稳态是决定细胞黏附、细胞壁硬度和弹性以及器官形态发生的关键因素,同时也是细胞壁应对逆境、释放抗性信号和免疫防御的分子模式。主要对PMEIs在调节植物器官发育过程和应对不同胁迫因子发挥的抗逆功能及调控机制等最新研究进展作出综述。鉴于PMEIs在木本植物中的体内生理活性和调控机制仍有待探索,可为后续填补该领域的研究空白提供理论依据和策略参考。
The cell wall is a complex and dynamic network structure, playing a vital regulatory role in plant growth and development, stress response, and immune resistance. As the main polysaccharide component in the primary cell wall structure, pectin is composed of homogalacturonic acid(HG), the most abundant linear polymer. The degree of methyl esterification of HG leads to its enzymatic hydrolysis to form a gel, thus affecting the stability of the pectin structure. Depending on post-translational mechanisms, pectin methylesterase inhibitors(PMEIs) regulate the PME activity by fine-tuning the balance of methyl esterification of pectin polysaccharides to maintain cell wall integrity and biomechanical properties. An increasing number of studies have shown that PMEI-PME interaction modulates the homeostasis of pectin methyl esterification, which is a crucial factor determining cell adhesion, cell wall porosity, elasticity, and organ morphogenesis. It is also an associated molecular pattern of cell wall-mediated immune response to environmental cues by releasing the anti-stress signals. This review presents the recent research progress on the physiological importance of PMEIs in regulating plant growth and development, especially regulatory mechanisms underlying the function of the stress tolerance in response to various stress stimuli. Since much remains unknown concerning the in vivo activity and regulatory mechanisms of PMEIs in woody plants, this review provides a theoretical basis and strategic reference for future research.
作者
熊媛
任襄襄
朱慧
任昕宜
潘子阳
苏涛
XIONG Yuan;REN Xiang-xiang;ZHU Hui;REN Xin-yi;PAN Zi-yang;SU Tao*()(Co-Innovation Center for Sustainable Forestry in Southern China,College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2022年第8期99-108,共10页
China Biotechnology
