摘要
为了研究红树杨叶肖槿来源内生真菌Talaromyces sp.YX-001的次级代谢产物及其乙酰胆碱酯酶(acetylcholinesterase,AChE)抑制活性,该文运用硅胶柱层析和HPLC等手段从菌株YX-001的PDB培养基发酵提取物中分离得到单体化合物,而后结合1/2D NMR和MS等现代波谱技术并与文献数据对比鉴定其结构,最后通过比色法测定各单体化合物的AChE抑制活性。结果表明:(1)共分离得到9个单体化合物,分别为asterrelenin(1),aszonalenin(2),cladosporisteroid C(3),sitosterol(4),ergosterol(5),cyclo-Ile-Pro-diketopiperazine(6),cyclo(-Pro-Val)(7),4-methoxy-2-methylisoquinolin-1-one(8)和allantoin(9)。(2)其中化合物1和2显示出一定的AChE抑制活性,IC_(50)值分别为81.5、105.8μmol·L^(-1)。该文首次对杨叶肖槿来源内生真菌次级代谢产物及其AChE抑制活性进行了研究,为后续红树植物杨叶肖槿来源内生真菌资源的开发与再利用奠定了基础。
In order to study on secondary metabolites and their acetylcholinesterase(AChE)inhibitory activities of the endophytic fungus Talaromyces sp.YX-001 derived from mangrove Thespesia populnea.The strain was fermented and cultured with 2%salinity PDB medium,and then subjected to silica gel column chromatography,semi-preparative HPLC,Sephadex LH-20,ODS and recrystallization.Nine compounds were identified using modern spectral techniques such as MS and 1/2D NMR,and comparing with literature data.All isolates were evaluated for their AChE inhibitory activities using ellman’s colorimetric method.The results were as follows:(1)Nine compounds were identified as asterrelenin(1),aszonalenin(2),cladosporisteroid C(3),sitosterol(4),ergosterol(5),cyclo-Ile-Pro-diketopiperazine(6),cyclo(-Pro-Val)(7),4-methoxy-2-methylisoquinolin-1-one(8),and allantoin(9).(2)The activities results showed that compounds 1 and 2 displayed moderate acetylcholinesterase inhibitory activities with IC_(50) values of 81.5 and 105.8μmol·L^(-1),while other compounds did not display significant inhibitory activities(IC_(50)>200μmol·L^(-1)).In this paper,the secondary metabolites and AChE inhibitory activities of endophytic fungi derive from T.populnea were studied for the first time,which lay a solid foundation for the subsequent development of endophytic fungi resources from T.populnea.
作者
薛欣怡
张翼
冯昀铠
廖清楠
胡雪琼
刘亚月
XUE Xinyi;ZHANG Yi;FENG Yunkai;LIAO Qingnan;HU Xueqiong;LIU Yayue(College of Food Science and Technology,Guangdong Ocean University,Zhanjiang 524088,Guangdong,China;Center for Marine Drugs Research&Development,Shenzhen Institute of Guangdong Ocean University,Shenzhen 518120,Guangdong,China;Collaborative Innovation Center of Seafood Deep Processing,Dalian Polytechnic University,Dalian 116034,Liaoning,China)
出处
《广西植物》
CAS
CSCD
北大核心
2022年第9期1487-1497,共11页
Guihaia
基金
国家自然科学基金(21807015)
广东海洋大学博士启动项目(R18008)
湛江市科技发展专项资金竞争性分配项目(2020A01030)
广东省扬帆计划引进紧缺拔尖人才项目(201433009)
深圳市科创委基础研究面上项目(JCYJ20190813105005619)
深圳市大鹏新区科技研发项目(KJYF202001-07)
广东省普通高校重点领域专项(生物医药与健康)(2021ZDZX2064)
广东省科技专项资金(2021A50114)
湛江市海洋经济创新发展示范市建设项目(湛海创XM-202008-01B1)。
关键词
杨叶肖槿
红树内生真菌
次级代谢产物
AChE抑制活性
海洋天然产物
Thespesia populnea
mangrove endophytic fungus
secondary metabolites
AChE inhibitory activity
marine natural product
