摘要
目的了解2009—2019年上海市闵行区手足口病的病原谱,分析肠道病毒71型(enterovirus 71,EV-A71)和柯萨奇病毒A组16型(coxsackievirus A16,CV-A16)VP1区基因特征,为手足口病的综合防治提供科学依据。方法对2009—2019年闵行区手足口病监测点送检的标本应用实时荧光定量PCR进行病原学检测,分析病原学特征。EV-A71和CV-A16病毒分离株进行VP1区核苷酸序列测定,分析其同源性并构建系统进化树。结果2009—2019年共收集到5364例手足口病病例标本,病原学检测阳性检出率为86.74%(4653/5364),其中EV-A71检出率为40.12%(2152/5364)、CV-A16为16.61%(891/5364)、CV-A6为20.86%(1119/5364)、CV-A10为1.49%(80/5364)。2009—2014年主要呈EV-A71和CV-A16共同流行趋势,2015—2019年主要以CV-A6和CV-A16共同流行为主。EV-A71分离株均为C4a型,VP1区核苷酸序列同源性为92.1%~99.3%、氨基酸序列同源性为98.1%~100.0%;CV-A16分离株均为B1基因型,存在B1b和B1a基因亚型的共同流行,VP1区核苷酸序列同源性为87.2%~99.6%、氨基酸序列同源性为97.9%~100.0%。结论2009—2019年上海市闵行区手足口病呈现EV-A71、CV-A16和CV-A6共同流行态势,不同年份的优势毒株呈现动态变化;而EV-A71流行株属于C4a亚型、CV-A16流行株存在B1b和B1a基因亚型的共同流行,与国内大部分地区的流行株基因亚型一致。
Objective To understand the etiological spectrum of hand,foot and mouth disease(HFMD)and analyze the VP1 genetic characteristics of enterovirus A group 71(EV-A71)and coxsackievirus group A 16(CV-A16)in Minhang District of Shanghai from 2009 to 2019,and to provide a scientific basis for comprehensive prevention and treatment of HFMD.Methods Real time PCR was used to detect HFMD specimens from surveillance hospitals in Minhang District during 2009-2019,and the pathogenic characteristics were analyzed.VP1 region nucleotide sequences of EV-A71 and CV-A16 isolates were determined,the homology was analyzed and the phylogenetic tree constructed.Results A total of 5,364 specimens of HFMD cases were collected during 2009-2019.Etiological detection revealed that the positive detection rate was 86.74%(4,635/5,364),of which the detection rates of EV-A71,CV-A16,CV-A6 and CV-A10 were 40.12%(2,152/5,364),16.61%(891/5,364),20.86%(1,119/5,364)and 1.49%(80/5,364),respectively.It mainly showed the common epidemic trend of EV-A71 and CV-A16 during 2009-2014,while during 2015-2019 it was mainly the common epidemic trend of CV-A6 and CV-A16.EV-A71 isolates were all C4a sub-genotype,and the sequences analysis displayed that EV-A71 strains shared 92.1%-99.3%nucleotide similarity and 98.1%-100.0%amino acid similarity with each other.CV-A16 isolates were all B1 genotypes,and there was a common prevalence of B1b and B1a sub-genotypes,the sequences analysis revealed that CV-A16 strains shared 87.2%-99.6%nucleotide similarity and 97.9%-100.0%amino acid similarity with each other.Conclusion The prevalent pathogens of HFMD in Minhang District during 2009-2019 were EV-A71,CV-A16 and CV-A6,and the dominant strains in different years showed dynamic changes.The EV-A71 strains belonged to C4a sub-genotype,and the CV-A16 strains had the common prevalence of B1b and B1a sub-genotypes.They were basically consistent with the sub-genotypes of epidemic strains in most parts of China.
作者
闫红静
甄珊珊
付贝尔
王之晗
王小光
张颖华
YAN Hong-jing;ZHEN Shan-shan;FU Bei-er;WANG Zhi-han;WANG Xiao-guang;ZHANG Ying-hua(Minhang District Center for Disease Control and Prevention,Shanghai 201101,China)
出处
《实用预防医学》
CAS
2022年第10期1158-1162,共5页
Practical Preventive Medicine
基金
上海市闵行区自然科学研究课题(2019MIIZ011)
闵行区公共卫生重点学科建设(MGWXK08)。
