摘要
目的:利用紫外分光光度法对六堡茶中茶色素含量进行测定。方法:取多个不同批次等级1级的六堡茶样品混合后制备茶色素对照品,通过顶空-气相色谱质谱法检测残留溶剂的量来进行茶色素的纯度标定,并利用标定的茶色素对照品制备标准曲线,通过测定不同六堡茶样品的吸光度,用标准曲线法计算出不同六堡茶样品中茶色素的含量。结果:本方法重复性试验的相对标准偏差为2.3%~4.2%;当加标浓度为0.5%~15.0%时,回收率为92.6%~106.2%;在浓度为25.66~128.30μg·mL^(-1)时线性相关系数良好,r=0.997。结论:该方法准确、可靠,适用于六堡茶中茶色素含量的测定。
Objective:To establish a ultraviolet spectrophotometry method for the tea pigments content in Liupao tea.Method:Mix multiple batches grade one Liupao tea samples which take as tea pigments reference substance,headspace-gas chromatography-mass spectrometry(HS-GC-MS)method was used to detect the residual solvent for calibrating the purity of tea pigments,then the standard curve was drawn against tea pigments standard which was calibrated.Through the detection of the absorbance of different Liupao tea samples,tea pigments calculate by standard curve method.Result:The relative standard deviations of reproducibility test of the methods were 2.3%~4.2%;when the spiked concentrations were 0.5%~15.0%,recovery rates were 92.6%~106.2%;when the concentration ranged from 25.66μg·mL^(-1)to 128.30μg·mL^(-1),the linear correlation coefficient was good,r=0.997.Conclusion:This method is accurate,reliable,which is suitable for the determination of tea pigments in Liupao tea.
作者
罗达龙
王华
覃蓝
蒋德莉
文俊萍
杨梓莹
宋妤
LUO Dalong;WANG Hua;QIN Lan;JIANG Deli;WEN Junping;YANG Ziying;SONG Yu(Wuzhou Institute for Food and Drug Control,Wuzhou 543000,China;Wuzhou Tianyu Tea Industry Co.,Ltd.,Wuzhou 543000,China;Guangxi Engineering Research Center of Instant Liupao Tea,Wuzhou 543000,China)
出处
《食品安全导刊》
2022年第26期59-61,65,共4页
China Food Safety Magazine
基金
广西科技重大专项(桂科AA20302018-15)
广西速溶六堡茶工程技术研究中心。
关键词
六堡茶
茶色素
顶空-气相色谱质谱法
紫外分光光度法
Liupao tea
tea pigments
headspace-gas chromatography-mass spectrometry
ultraviolet spectrophotometry
