摘要
目的 完善优化术苓健脾胶囊的薄层色谱鉴别方法。方法 将术苓健脾胶囊内容物依次用正己烷和甲醇超声提取,提取液分别用于制剂中白术的鉴别和制剂中白芍、黄柏和陈皮的鉴别,取部分甲醇提取液过滤、蒸干,加水溶解,通过D101型大孔吸附树脂柱(内径2 cm,树脂高15 cm),分别用10%甲醇、50%甲醇、80%甲醇和90%甲醇依次洗脱,取50%甲醇洗脱液,用于制剂中乌药和防风的鉴别;取80%甲醇洗脱液,用于制剂中甘草的鉴别;取90%甲醇洗脱液,用于制剂中厚朴的鉴别。结果 一次取样制备5份供试品溶液,用于制剂中8味药材的薄层色谱鉴别。白芍以芍药苷为指标,乌药以去甲异波尔定为指标,防风以升麻素苷和5-O-甲基维斯阿米醇苷为指标,均以氯仿-甲醇(4∶1)为展开剂;黄柏以盐酸小檗碱为指标,陈皮以橙皮苷为指标,均以氯仿-甲醇-水(37∶17∶5)的下层溶液为展开剂;厚朴以厚朴酚及和厚朴酚为指标,甲苯-甲醇(10∶1)为展开剂,甘草以甘草苷和甘草酸单铵盐为指标,乙酸乙酯-甲酸-冰醋酸-水(15∶1∶1∶2)为展开剂。防风、乌药和甘草为新增薄层色谱鉴别项,厚朴、黄柏和陈皮的薄层色谱鉴别项得到优化。结论 采用一次系统试验完成术苓健脾胶囊多味药材薄层色谱鉴别项的供试品溶液制备,多味药材使用相同展开剂,操作方法更加简便,重现性和分离度提高,更有助于术苓健脾胶囊的质量控制。
Objective Jianpi Capsule via successive ultrasonication with n-hexane following with mehanol were separately used for the identification of Atractylodis Macrocephalae Rhizoma and for the identifications of Paeoniae Radix Alba,Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium in Zhuling Jianpi Capsule. The residual methanol filtrate was evaporated to dryness and dissolved with water,applied to D101 macroporous resin column(inner diameter 2 cm,column length 15 cm),eluted successively with 10% methanol,50% methanol,80% methanol and 90% methanol. The 50%methanol eluate was used for identification of Linderae Radix and Saposhnikoviae Radix,80% methanol eluate was used for identification of Glycyrrhizae Radix et Rhizoma,and 90% methanol eluate for identification of Magnoliae Officinalis Cortex.Results methods were accomplished,and three TLC identification methods for Saposhnikoviae Radix,Linderae Radix and Glycyrrhizae Radix et Rhizomawere were newly established and three TLC identification methods for Magnoliae Officinalis Cortex,Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium were improved. Paeoniflorin was used as the marker of Paeoniae Radix Alba,norisoboldine as the marker of Linderae Radix,prim-Oglucosylcimifugin and 5-O-methylvisammioside as the markers of Saposhnikoviae Radix,and the mixture of chloroform and methanol(4∶1)was used as the same mobile phase for three of them;berberine hydrochloride was used as the marker of Phellodendri Chinensis Cortex and hesperidin as the ,-the mixture of chloroform,methanol and water(37∶17∶5)was used as the same mobile phase for both of them;Magnolol and honokiol were used as the markers of Magnoliae Officinalis Cortex and the mixture of toluene and methanol(10∶1) as the mobile phase;liquiritin and monoammonium glycyrrhizinate were used as the markers of Glycyrrhizae Radix et Rhizoma,and the mixture of ethyl acetate,formic acid,glacial acetic acid,and water(15∶1∶1∶2)as the mobile phase.ConclusionA systematic sample test solution pre
作者
方洪
陈夕梅
谷丽华
王煜
杨莉
黄素和
杨飞
陈天琦
王峥涛
FANG Hong;CHEN Ximei;GU Lihua;WANG Yu;YANG Li;HUANG Suhe;YANG Fei;CHEN Tianqi;WANGZhengtao(Shanghai R&D Center for Standardization of Chinese Medicines,Shanghai 201203,China;Key Laboratory of Standardization of Chinese Medicines,Ministry of Education,Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Fuzhou Meifeng Pharmaceutical Factory,Fuzhou,Fujian 350007,China)
出处
《上海中医药杂志》
2022年第7期102-106,共5页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市科委科研计划项目(19ZR1457200)。
关键词
肠易激综合征
术苓健脾胶囊
薄层色谱
质量标准
中药研究
irritable bowel syndrome
Zhuling Jianpi Capsule
TLC identification
quality standard
traditional Chinese herbal medicine research
