摘要
目的 建立党参和蜜麸炒党参配方颗粒超高效液相色谱(UPLC)指纹图谱和含量测定方法,并对其质量进行评价。方法 采用沃特世(Waters)ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm),以乙腈为流动相A,以0.1%甲酸水溶液为流动相B进行梯度洗脱,流速为0.3 mL/min,柱温为30℃,检测波长为268 nm,进样量为3μL。结果 建立了蜜麸炒党参配方颗粒UPLC指纹图谱和含量测定方法,党参炔苷在2.4~100.0 mg·L^(-1)范围内线性关系良好(R^(2)=0.999 7),加样回收率(50%、100%、150%)分别为97.72%、96.23%、98.60%,平均加样回收率为97.52%,RSD为1.76%。方法的精密度、重复性、稳定性试验RSD均小于3%。指纹图谱确定了9个共有峰,并指认了党参炔苷、党参苷Ⅰ、党参炔苷宁、党参炔醇4个色谱峰。28批党参和蜜麸炒党参配方颗粒的党参炔苷含量介于0.06~0.39 mg·g^(-1)。根据主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)结果能明显区分党参和蜜麸炒党参配方颗粒,且不同企业的党参配方颗粒存在一定的差异。结论 该法操作简便、检测效率高、专属性强,能同时用于蜜麸炒党参配方颗粒和党参配方颗粒的质量评价。
Objective honey bran frying Codonopsis Radix dispensing granules(HBFCRDG),and to provide a comprehensive evaluation of the quality of CRDG and HBFCRDG.MethodsUPLC analysis was performed on a Waters ACQUITY UPLC HSS T3 column(2.1 mm×100 mm,1.8μm),with a mobile phase consisting of acetonitrile(A)-0.1%formic acid(B)at the flow rate of 0.3 mL·min^(-1).The column temperature was controlled at 30℃and the detection wavelength was set at 268 nm.The injection volume was 3μL.ResultsUPLC fingerprint and content determination methods of HBFCRDG were established.Lobetyolin displayed ideal linearity within the range of 2.4-100.0 mg·L^(-1)(R^(2)=0.9997).The recoveries of lobetyolin in three levels of50%,100%and 150%were 97.72%,96.23%,98.60%respectively,and the total average recovery was 97.52%with RSD of 1.76%.The RSD values of precision,repeatability and stability were all less than 3%.Nine common peaks were designated in fingerprint and peaks of lobetyolin,tangshenosideⅠ,lobetyolinin and lobetyol were confirmed by standard references.The content of lobetyolin in 28 batches of CRDG and HBFCRDG was between0.06-0.39 mg·g^(-1).According to the results of PCA and PLS-DA,HBFCRDG can be distinguished from CRDG obviously.What’s more,it showed that manufacturers.ConclusionThe method has the advantages of simple operation,high detection efficiency and strong specificity,and can be used for the quality evaluation of CRDG and HBFCRDG.
作者
陈倩萍
谢琦
党蕊
孙鑫
安定邦
程雪梅
李文艳
王长虹
CHEN Qianping;XIE Qi;DANG Rui;SUN Xin;AN Dingbang;CHENG Xuemei;LI Wenyan;WANG Changhong(Institute of Chinese Materia Medica,Shanghai Key Laboratory of Compound Chinese Medicines,The MOE Key Laboratory for Standardization of Chinese Medicines,Shanghai Research Center for Standardization of Chinese Medicine,Shanghai Universityof Traditional ChineseMedicine,Shanghai201203,China)
出处
《上海中医药杂志》
2022年第7期83-88,共6页
Shanghai Journal of Traditional Chinese Medicine
基金
科技部国家重点研发计划“中医药现代化研究重点专项”(2018YFC1706304)
上海市科委“科技创新行动计划”技术标准项目(21DZ2202200)
上海市卫健委中医药传承创新发展三年行动计划建设项目(ZY[2021-2023]-0215)。
关键词
党参
蜜麸炒党参
配方颗粒
超高效液相色谱
中药研究
Codonopsis Radix
honey bran frying Codonopsis Radix
dispensing granules
UPLC
traditional Chinese herbal medicine research
