摘要
In the present study,we aimed to investigate the effects of Polygonum hydropiper aqueous extract(PHE)on the expression levels of inflammatory cytokines and cytochrome P450(CYPs)in mice with E.coli-induced diarrhea.BALB/c mice were randomly divided into the control group,model group,enrofloxacin-treated group,and two PHE-treated groups with different doses.The diarrhea model was established by intraperitoneal injection of enteropathogenic E.coli(EPEC)in mice.The enrofloxacin-treated group was given enrofloxacin at 5 mg/kg by intragastric gavage(i.g.)for 11 d.PHE-treated groups were given PHE at 5 g/kg and 10 g/kg by i.g.for 11 d.The histopathological characteristics of the duodenum and liver were observed by HE staining.The levels of inflammatory cytokines and CYPs in the duodenum and liver of mice were determined by ELISA.The m RNA and protein expressions of CYPs were determined by q RT-PCR and Western blotting analysis,respectively.The results showed that PHE could significantly alleviate the injury of the duodenum and liver induced by EPEC infection,reduce the contents and m RNA expressions of inflammatory cytokines,and regulate the m RNA and protein expressions of the major subtypes of CYPs.These findings indicated that PHE had an apparent therapeutic effect on EPEC-induced diarrhea,and its mechanism might be related to inhibition of inflammatory cytokines and regulation of CYPs.
本文旨在探讨辣蓼水提液对大肠杆菌性腹泻小鼠炎症因子和细胞色素P450(CYPs)酶表达的影响。将BALB/c小鼠随机分为正常组、模型组、恩诺沙星组、辣蓼水提液5g/kg组和10g/kg组。腹腔注射致病性大肠杆菌建立小鼠腹泻模型,于造模前两天各组给予相应药物灌胃,模型组和正常组给予等量的生理盐水,连续给药11天。HE染色法观察十二指肠和肝脏的病理组织学特征,ELISA法检测十二指肠和肝脏中炎症因子和CYPs主要亚型的含量,q RT-PCR和Western Blot法检测CYPs的m RNA及蛋白表达。结果表明,辣蓼水提液可明显缓解致病性大肠杆菌诱导的小鼠肠道及肝脏损伤,降低炎症因子的含量及m RNA表达,对CYPs主要亚型的m RNA和蛋白表达水平有明显的调节作用。辣蓼对大肠杆菌性腹泻有明显的治疗作用,其机制可能与抑制炎症因子释放、调节药物代谢酶CYPs的表达有关。
基金
Key Project at Central Government Level:The ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302-2004-03)。
