摘要
【目的】探究微塑料和多环芳烃对农用地土壤微生物群落结构、生物学功能和代谢途径的影响,为微塑料-多环芳烃复合污染的生态风险评估和修复提供理论依据。【方法】选用聚氯乙烯、芘作为微塑料和多环芳烃的代表,利用宏基因组学技术结合PICRUSt功能预测研究聚氯乙烯-芘复合污染胁迫下农用地土壤微生物群落丰度、组成和功能的响应。【结果】添加聚氯乙烯和芘后土壤微生物群落结构发生了变化,Acidobacteria、Betaproteobacteria、Gemmatimonas、Nitrososphaera和Burkholderia等的相对丰度降低,Sphingomonas、Nocardioides、Lysobacter、Marmoricola、Streptomyces、Mycobacterium、Sphingopyxis、Sphingobium、Novosphingobium、Luteimonas等的相对丰度上升;基于GO功能、KEGG代谢通路和CAZy酶功能分析表明,聚氯乙烯-芘可以增强土壤微生物的发育、繁殖和自我调节功能,加快菌群的代谢进程和对有害产物的转运/排泄过程。【结论】聚氯乙烯和芘的存在引起土壤微生物群落结构和功能的改变,提高了其自身的抗氧化能力,促使微生物对污染环境具有更好的适应能力。
【Objective】The study was conducted to explore the effects of microplastics and polycyclic aromatic hydrocarbons(PAHs) on microbial community structure,biological functions and metabolic pathways in agricultural soil so as to provide a theoretical basis for ecological risk assessment and remediation of combined microplastics-PAHs pollution.【Method】Polyvinyl chloride(PVC) and pyrene(PY) were selected as the representatives of microplastics and PAHs,respectively.The responses of abundance,composition and function of microbial communities in agricultural soil to the combined pollution of PVC-PY were studied by using metagenomic techniques and PICRUSt function prediction.【Result】Modifications of soil microbial community structure were found with the addition of PVC and PY.The relative abundance of Acidobacteria,Betaproteobacteria,Gemmatimonas,Nitrososphaera and Burkholderia were decreased in the presence of PVC-PY.Addition of PVC-PY improved the relative abundance of Sphingomonas,Nocardioides,Lysobacter,Marmoricola,Streptomyces,Mycobacterium,Sphingopyxis,Sphingobium,Novosphingobium and Luteimonas.According to the analysis of GO function,KEGG metabolic pathway and CAZy enzyme function,PVC-PY showed positive effects on the development,reproduction and self-regulation of soil microorganisms,and then accelerated the metabolic process of bacteria flora and the transport/excretion process of harmful products.【Conclusion】The addition of PVC-PY induced the changes in the structure and function of soil microbial communities,which increased their antioxidant capacity.Thus,the soil microbes had better adaptability to polluted environment.
作者
刘沙沙
操江飞
陈显宇
梁绮彤
LIU Shasha;CAO Jiangfei;CHEN Xianyu;LIANG Qitong(School of Environmental and Chemical Engineering,Zhaoqing University/Guangdong Key Laboratory of Environmental Health and Land Resource,Zhaoqing 526061,China)
出处
《广东农业科学》
CAS
2022年第8期64-72,共9页
Guangdong Agricultural Sciences
基金
国家自然科学基金(42007317)
广东省基础与应用基础研究基金(2019A1515110272)
肇庆学院优秀青年教师科研能力提升计划项目(YQ202105)
国家大学生创新创业训练计划项目(202210580015)。
关键词
聚氯乙烯
芘
农用地土壤
微生物群落结构
代谢通路
碳水化合物活性酶
polyvinyl chloride
pyrene
agricultural soil
microbial community structure
metabolic pathway
carbohydrate active enzyme
