摘要
以香菇为试验原料,采用双酶耦合超高压提取香菇多糖,在单因素实验基础上,经响应面优化其提取工艺,并研究其抗氧化活性。结果表明:最佳提取工艺条件为双酶(纤维素酶:木瓜蛋白酶,质量比为1∶2)添加量2.5%,酶解pH5.5,压力450MPa,在此条件下,香菇多糖提取率为8.83%。香菇多糖对DPPH自由基和羟自由基有一定的清除能力,当其浓度为25mg/mL时,对DPPH自由基和羟自由基的清除率分别为79.62%和81.59%。本研究提供一种新的香菇多糖提取方法。
Lentinus edodes was used to extract lentinan(LNT)by double enzyme coupling ultra high pressure extraction method. The extraction process was optimized by response surface methodology on the basis of single factor experiment. The antioxidant activity was also studied. The results showed that the optimum extraction conditions were :the amount of double enzyme(cellulase :papain,mass ratio 1∶2)2.5 %,pH of enzymatic hydrolysis 5.5and reaction pressure 450 MPa. Under these conditions,the extraction rate of LNT was 8.83 %. LNT had a certain scavenging capacity for DPPH radicals and hydroxyl radicals. When the concentration of LNT was 25mg/mL,the scavenging rates of DPPH radical and hydroxyl radical were 79.62 % and 81.59 % respectively. This study provides a new extraction method for LNT.
作者
张彩芳
秦令祥
周婧琦
赵俊芳
ZHANG Caifang;QIN Lingxiang;ZHOU Jingqi;ZHAO Junfang(Luohe Vocational College of Food,Luohe 462300;Luohe Food Research Institute Co.,Ltd.,Luohe 462300)
出处
《中国食品添加剂》
CAS
北大核心
2022年第9期69-74,共6页
China Food Additives
基金
河南省科技攻关项目(182102110005)
漯河市重大科技创新专项项目(20210109)。
关键词
香菇
香菇多糖
超高压
双酶
工艺优化
抗氧化活性
Lentinus edodes
lentinan
ultra-high pressure
double enzyme
process optimization
antioxidant activity
