摘要
纳米科学技术的快速发展为食品相关纳米粒子(food-related nanoparticles,FNP)的合成提供了新方法,这些FNP在食品科学的研究和应用方面具有巨大潜力。除了人工合成的用于食品添加剂的FNP,在食品加工过程发现的内源性FNP使研究其在体内的安全性变得更加重要。研究发现,FNP在体液运输过程中会因吸附蛋白质而在表面形成蛋白冠,蛋白冠影响FNP的理化性质以及蛋白质的结构和功能,进而产生一系列生物反应。本文综述了FNP蛋白冠的基本情况,阐述了蛋白冠对FNP性质、蛋白质结构及功能的影响,以及蛋白冠对FNP细胞内化、细胞毒性和免疫反应的影响。了解蛋白冠的影响对于评估FNP的生物效应以及蛋白质和FNP之间相互作用的物理、化学和生物信息有重要意义。本文较为系统地评价了食品中纳米颗粒形成蛋白冠的安全性,为了解和控制食品相关纳米毒性提供了基础信息,并对今后食品相关纳米粒子蛋白冠的研究方向进行了展望。
The rapid development of nanoscience and technology provides a new method for the synthesis of food-related nanoparticles(FNP),the FNP has great potential in the research and application of food science.In addition to the artificially synthesized FNP used in food additives,the discovery of endogenous food-borne nanoparticles generated during food processing has made it more important to study the safety of FNP in the body.Studies have found that FNP can adsorb proteins during the transportation of body fluids,forming protein corona on the surface.Protein corona affects the physical and chemical properties of FNP and the structure and function of proteins,which in turn affects a series of biological reactions.This article reviewed the basic situation of FNP protein corona,expounded the effects of protein corona on the properties,protein structure and function of FNP,and the effects of protein corona on FNP cellular internalization,cytotoxicity and immune response.Understanding the influence of protein corona was of great significance for evaluating the biological effects of FNP and physicochemical and biological information about the interaction between proteins and FNP.Finally,the future research directions of food-related nanoparticles protein corona were prospected.This paper will systematically evaluate the safety of protein coronas formed by nanoparticles in food,and provide basic information for understanding and controlling food-related nanotoxicity.
作者
谭明乾
刘荣刚
崔国馨
宋玉昆
TAN Mingqian;LIU Ronggang;CUI Guoxin;SONG Yukun(Academy of Food Interdisciplinary Science/School of Food Science and Technology/National Engineering Research Center of Seafood,Dalian Polytechnic University,Dalian 116033,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2022年第5期1041-1050,共10页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(31872915)
中央引导地方科技发展专项资金(2020JH6/10500002)。
关键词
纳米粒子
蛋白冠
相互作用
细胞毒性
细胞内化
nanoparticles
protein corona
interaction
cytotoxicity
cellular internalization
