摘要
目的观察益生菌对帕金森病伴抑郁小鼠5-羟色胺及相关炎性因子水平的影响,探讨帕金森病伴抑郁的发病机制及治疗新思路。方法选取50只C57/BL6成年雄性小鼠,采用随机数字表法分为单纯抑郁组(抑郁组)、单纯帕金森病组(PD组)、帕金森病伴抑郁组(PDD组)、益生菌治疗组(治疗组)和正常对照组,各10只。采用1-甲基-4-苯基-1,2,3,6-四氢吡啶腹腔注射方法制备单纯帕金森病小鼠模型,慢性不可预知温和应激建立单纯抑郁小鼠模型。上述2种方法结合建立帕金森病伴抑郁小鼠模型,PDD组和治疗组小鼠均为此模型。模型制备成功后,治疗组给予益生菌灌胃,正常对照组正常饲养、不给予任何干预,抑郁组、PD组及PDD组未予药物干预。检测小鼠海马5-羟色胺水平、外周血辅助性T细胞17(Th17)细胞占比及黑质纹状体中白细胞介素17A(IL-17A)、肿瘤坏死因子α(TNF-α)及维甲酸相关核孤儿受体(ROR-γt)表达量。结果抑郁组、PD组、PDD组和治疗组海马5-羟色胺水平均明显低于正常对照组[(97.6±3.6)、(83.7±4.2)、(79.0±3.8)、(95.2±2.0)μg/L比(114.4±4.5)μg/L],但治疗组明显高于PDD组(均P<0.05)。抑郁组、PD组、PDD组和治疗组小鼠外周血Th17细胞占比及黑质纹状体中IL-17A、TNF-α、ROR-γt表达量均明显高于正常对照组,但治疗组均明显低于PDD组(均P<0.05)。结论益生菌可升高帕金森病伴抑郁小鼠5-羟色胺水平,抑制Th17及其相关炎性因子IL-17A、TNF-α和ROR-γt的表达。
Objective To observe the effects of probiotics on the levels of 5-hydroxytryptamine and related inflammatory factors in mice with Parkinson′s disease and depression,and to explore the pathogenesis and treatment of Parkinson′s disease and depression.Methods Totally 50 C57/BL6 adult male mice were randomly divided into simple depression group(depression group),simple Parkinson′s disease group(PD group),Parkinson′s disease complicated with depression group(PDD group),probiotic treatment group(treatment group)and normal control group,with 10 in each group.Intraperitoneal injection of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine to prepare the mouse model of simple Parkinson′s disease,and the mouse model of depression was established by chronic unpredictable mild stress.The above two methods were combined to establish the mouse model of Parkinson′s disease complicated with depression,and mice in PDD group and treatment group were this model.After successful model preparation,the treatment group was given probiotics intragastric administration,the normal control group was fed normally without intervention.The depression group,PD group and PDD group were not given drug intervention.The level of 5-hydroxytryptamine in hippocampus,the rate of helper T cell(Th17)in peripheral blood,expressions of interleukin-17 A(IL-17 A),tumor necrosis factor-α(TNF-α)and retinoic acid related nuclear orphan receptor(ROR-γt)in substantia nigra and striatum were detected.Results The levels of 5-hydroxytryptamine in hippocampus in depression group,PD group,PDD group and treatment group were lower than that in normal control group[(97.6±3.6),(83.7±4.2),(79.0±3.8),(95.2±2.0)μg/L vs(114.4±4.5)μg/L],while the level in treatment group was higher than that in PDD group(all P<0.05).The rate of Th17 in peripheral blood and expressions of IL-17 A,TNF-αand ROR-γt in substantia nigra and striatum in depression group,PD group,PDD group and treatment group were higher than those in normal control group and above indexes in treatme
作者
刘斌
李炳翰
任伯
范少凯
张艳淑
吴小坤
邓春颖
Liu Bin;Li Binghan;Ren Bo;Fan Shaokai;Zhang Yanshu;Wu Xiaokun;Deng Chunying(First Department of Neurology,North China University of Science and Technology Affiliated Hospital,Tangshan 063000,China;Laboratory Animal Center,North China University of Science and Technology,Tangshan 063000,China)
出处
《中国医药》
2022年第9期1361-1365,共5页
China Medicine
基金
河北省政府资助临床医学优秀人才培养项目计划(2019046)。
关键词
益生菌
帕金森病
抑郁
5-羟色胺
炎性因子
Probiotics
Parkinson′s disease
Depression
5-hydroxytryptamine
Inflammatory factor
