摘要
糖基化是生物体内重要的蛋白质翻译后修饰(PTM)。基于质谱的检测技术,特别是液相色谱-质谱(LC-MS)联用技术是糖基化研究的常规手段。然而,由于糖基化存在复杂的异构现象,大多数基于质谱的分析技术在区分其异构体时存在困难。离子迁移谱(IM)是一种基于离子在气相中的形状和电荷进行分离的技术,能够为质谱分析提供新的分离维度。离子迁移谱-质谱(IM-MS)技术具有较强的区分异构体的能力,在蛋白质糖基化研究中的潜力越来越受到重视。本文总结了IM-MS技术的类型和基本原理,及其在蛋白质糖基化研究中的进展,如对完整糖蛋白、完整糖肽和聚糖的研究,并展望了其应用前景。
Glycosylation is one of the most important post-translational modifications(PTM),mediating physiological processes like cell adhesion,cell proliferation,cell signaling,and immune responses.The special functionality of glycosylation for cancer progression and metastasis were reported in recent researches.Analytical methods based on mass spectrometry(MS),especially liquid chromatography mass spectrometry(LC-MS),are the routine practices for protein glycosylation research.However,due to the high complexity and multiformity of glycan structures,as well as the existence of numerous isomers,in-depth analysis of glycosylation ends up as a major challenge for conventional LC-MS-based method.Ion mobility separation is a promising approach to fill the gap as it provides an additional separation dimension for mass spectrometry analysis based on structures and charges of the gas-phase ions.As a technology with an entirely different separation mechanism from that of liquid chromatography,ion mobility spectrometry(IM)has several properties superior to other separation facilities.First of all,unlike liquid chromatography or capillary electrophoresis which demand minutes of separation time,IM only requires a few milliseconds to finish the separation,and it is well compatible with fast mass spectrometry analysis.Besides,as a separation technique for gas phase ions,IM can be modularized and readily integrated into full-fledged LC-MS system.Most importantly,IM is proved to have great potential in glycan isomer discriminations.Given the aforementioned merits,ion mobility mass spectrometry(IM-MS)is gaining attention from glycomics community.This review summarized the basic principles of IM-MS and its applications in protein glycosylation research for the past five years.The means of enhancing isomeric separation performance of glycans were comprehensively discussed,including derivatization,forming metal and other noncovalent adducts,and utilizing high-resolution ion mobility spectrometry implementation.The various approaches of lever
作者
付彬
梁宇颖
陆豪杰
FU Bin;LIANG Yu-ying;LU Hao-jie(Institutes of Biomedical Sciences and Department of Chemistry,Fudan University,Shanghai 200032,China;NHC Key Laboratory of Glycoconjugates Research,Fudan University,Shanghai 200032,China)
出处
《质谱学报》
EI
CAS
CSCD
北大核心
2022年第5期564-579,I0004,共17页
Journal of Chinese Mass Spectrometry Society
基金
国家自然科学基金(21974025)。
