摘要
为探究老班瑶药牛耳风叶绿体基因组的结构特征、系统进化以及密码子偏好性,以瑶药牛耳风为研究材料,对其叶绿体基因组进行测序和组装。结果表明,牛耳风叶绿体基因组由大单拷贝区、小单拷贝区以及1对反向重复区组成,全长189 920 bp,包含118个基因,其中99个编码蛋白质的基因,11个tRNA基因,8个核糖体rRNA基因;共检测到71个简单序列重复(simple sequence repeat,SSR)位点。系统进化分析表明牛耳风与番荔枝属的刺果番荔枝(Annona muricata)亲缘关系最近。密码子偏好性分析表明,牛耳风的密码子偏好性较弱,且密码子偏好性主要受选择因素的影响,最终选出11个最优密码子,其中10个以A/U结尾,为瑶药牛耳风的系统进化研究等提供科学依据。
To study the structure characteristics,phylogeny and codon usage bias of chloroplast genome in Fissisfigma polyanthum Merr.,the chloroplast genome of Fissisfigma polyanthum Merr. was sequenced and analyzed.The results showed that the chloroplast genome was composed of large single-copy region,small single-copy region and a pair of reverse repeat regions. The total length was 189 920 bp,which included 118 genes(99 genes encoding protein,11 tRNA genes and 8 ribosome rRNA genes),and 71 simple sequence repeats(SSRs)were also identified.The phylogenetic analysis showed that F. polyanthum Merr. was closely related to Annona muricata. The codon preference analysis showed that the codon usage bias of F. polyanthum Merr. was weak,and the codon preference was mainly affected by selection factor. Finally,11 optimal codes were selected,10 of which ended with A/U. These results provided scientific basis for phylogeny and construction of expression vector of F. polyanthum Merr.
作者
严其伟
梁湘兰
陶秋云
罗秋香
郭松
YAN Qiwei;LIANG Xianglan;TAO Qiuyun;LUO Qiuxiang;GUO Song(College of Food and Biochemical Engineering,Guangxi Science&Technology Normal University,Guangxi Laibin 546119,China;Key Lab for Zhuang and Yao Pharmaceutical Quality Biology,Guangxi Science&Technology Normal University,Guangxi Laibin 546199,China;JinxiuYao Autonmous County State Owned Jinxiu Forert Form,Guangxi Laibin 545799,China;Yao Medical Hospital of Jinxiu Yao Autonomous County,Guangxi Laibin 545799,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2022年第8期74-86,共13页
Journal of Agricultural Science and Technology
基金
广西科技师范学院高层次人才启动经费项目(GXKS2020GKY009)
广西科技师范学院壮瑶药品质生物学重点实验室项目(GXKSKYPT2021007)
广西高校中青年教师科研基础能力提升项目(2021KY0862)。
关键词
牛耳风
叶绿体基因组
特征
密码子偏好性
Fissisfigma polyanthumMerr.
chloroplast genome
characteristics
codon usage bias
