摘要
背景:建立动物模型是研究卒中后吞咽障碍的重要研究方法之一,线栓法是缺血性脑卒中最常用的经典模型,新近研究表明,化学损毁吞咽中枢疑核也能造成大鼠表现吞咽功能障碍,但其造模成功率、临床相似性、可调控性仍不确定。目的:观察线栓法与化学损毁法两种不同造模方法对大鼠模型的吞咽功能改变,探究能较完整表现与人类相似的吞咽障碍临床及病理特征。方法:将40只SD大鼠随机分为3组,正常组10只,线栓组15只,化学损毁组15只,线栓组以线栓法短暂脑缺血90 min后再灌注制备左侧卒中脑缺血模型,化学损毁组以鹅膏蕈氨酸化学损毁疑核造成大鼠吞咽障碍模型。记录各组大鼠每周存活数、体质量及24 h进食、进水量,在造模后第2,7,14,30天时采用生物信号采集器及张力换能器检测大鼠吞咽启动反应时间及吞咽次数,并采用苏木精-伊红染色观察延髓吞咽中枢疑核处病理学形态变化,ELISA法观察各组大鼠血清的白细胞介素6、白细胞介素1β、肿瘤坏死因子α的表达变化。结果与结论:①和化学损毁组相比,线栓组大鼠死亡率在第2,7天显著降低(P<0.05),其余时间段无明显差异(P>0.05),且线栓组最终死亡率明显低于化学损毁组(40%,73%,P<0.05);②与正常组相比,造模后第7天化学损毁组大鼠体质量及24 h进食、进水量均减少、吞咽启动反应时间延长、吞咽次数减少,差异均有显著性意义(P<0.05),线栓组无明显差异(P>0.05);与正常组相比,第14天后2个造模组大鼠体质量及24 h进食、进水量均减少,吞咽启动反应时间均延长,吞咽次数均减少,差异均有显著性意义(P<0.05);线栓组和化学损毁组相比,大鼠体质量及24 h进食、进水量、吞咽启动反应时间、吞咽次数在第7天差异有显著性意义(P<0.05),其余时间段无明显差异(P>0.05);③2个造模组大鼠的延髓处均较出现明显病理性改变,延髓处炎症细胞�
BACKGROUND:The establishment of animal models is one of the important methods to study swallowing disorders after stroke.Suture-occluded method is the most commonly classic model of ischemic stroke.Recent studies have shown that the chemical destruction of the nucleus ambiguus can also cause swallowing disorders in rats.However,its modeling success rate,clinical similarity,and controllability remain uncertain.OBJECTIVE:To observe the changes in swallowing function in the rat model established by two different modeling methods,namely,suture-occluded method and chemical destruction method,and to investigate the clinical and pathological characteristics of swallowing disorders similar to those in humans.METHODS:Forty Sprague-Dawley rats were randomly divided into three groups:normal group(n=10),suture-occluded group(n=15),and chemical destruction group(n=15).The left-sided ischemic stroke models were prepared using the suture-occluded method through reperfusion after 90 minutes of transient cerebral ischemia in the suture-occluded group,while rat models of swallowing disorders were established through the chemical destruction of the nucleus ambiguus by gooseberry muscarinic acid in the chemical destruction group.Survival numbers per week,body mass,and 24-hour food and water intake in each group were recorded.The rats were examined on days 2,7,14,and 30 after modeling using a biosignal collector and a tension transducer to detect the swallowing initiation response time and the number of swallows.Hematoxylin-eosin staining was used to observe the pathomorphological changes of the nucleus ambiguus of the medulla oblongata swallowing center.ELISA method was used to detect the levels of serum interleukin-6,interleukin-1βand tumor necrosis factor-α.RESULTS AND CONCLUSION:The mortality rate in the suture-occluded group was significantly lower than that in the chemical destruction group on days 2 and 7(P<0.05),but there was no significant difference between the two groups at other observation time points(P>0.05).The final
作者
华晓琼
李彦杰
李斯锦
张淑芹
刘昊源
丁慧敏
赵楠楠
Hua Xiaoqiong;Li Yanjie;Li Sijin;Zhang Shuqin;Liu Haoyuan;Ding Huimin;Zhao Nannan(Rehabilitation School of Medicine,Henan University of Chinese Medicine,Zhengzhou 450046,Henan Province,China;Department of Rehabilitation,Henan Province Hospital of TCM,Zhengzhou 450046,Henan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第11期1708-1714,共7页
Chinese Journal of Tissue Engineering Research
基金
河南省科技攻关计划项目(222102310569),项目负责人:李彦杰
河南省中医药科学研究专项课题(重点课题)(2019ZYBJ14),项目负责人:李彦杰
河南省中医药科学研究专项课题(2022ZY1083),项目负责人:李彦杰
河南省中医药拔尖人才培养项目(豫中医科[2018]35号),项目负责人:李彦杰。
关键词
吞咽功能
化学损毁
疑核
脑缺血
动物模型
swallowing function
chemical destruction
nucleus ambiguus
cerebral ischemia
animal model
