摘要
基因扩增仪工作温度控制过程中,受到温度检测技术的影响,导致温度震荡幅值较大。因此,提出基于生物传感技术的基因扩增仪温度控制方法。针对基因扩增仪工作原理,构建PCR仪样品块稳态热模型。依托于生物传感技术设计SAW生物传感器,建立生物传感温度检测技术。利用PID控制器建立自适应温度控制方案,并对温度控制主要参数进行整定处理。实验结果表明:应用所提出的温度控制方法,温度震荡幅值为±1.3℃,满足基因扩增仪温度控制要求。
In the process of working temperature control of gene amplification instrument,affected by temperature detection technology,the final temperature oscillation amplitude is large.Therefore,a temperature control method of gene amplification instrument based on biosensor technology is proposed.According to the working principle of gene amplification instrument,the steady-state thermal model of PCR instrument sample block was constructed.Based on biosensor technology,saw biosensor is designed and biosensor temperature detection technology is established.The adaptive temperature control scheme is established by using PID controller,and the main parameters of temperature control are adjusted.The experimental results show that after the application of the proposed temperature control method,the temperature oscillation amplitude is 1.3℃,which meets the temperature control requirements of gene amplification instrument.
作者
雷亚若
王文毅
张翰章
陈建林
LEI Yaruo;WANG Wenyi;ZHANG Hanzhang;CHEN Jianlin(School of Laboratory Medicine,Chengdu Medical college,Chengdu 610500,China)
出处
《自动化与仪器仪表》
2022年第7期140-143,共4页
Automation & Instrumentation
基金
国家级大学生创新创业计划(201913705027)
四川省医学科研课题计划(Q18020)
四川省卫生和计划生育委员会科研项目(Q18PJ553)
四川养老与老年健康协同创新中心自然科学项目(19Z06)
四川省科技计划资助(2021JDRC0044)。
关键词
生物传感技术
基因扩增仪
温度控制
LOVE波
PID控制器
稳态热模型
biosensor technology
gene amplification instrument
temperature control
love wave
PID controller
steady state thermal model
