摘要
为了提高活疫苗的稳定性,便于活疫苗的保存和运输,本试验将猪乙型脑炎病毒分别与耐热冻干保护剂和传统冻干保护剂进行配比、冻干,制备活疫苗,分别将疫苗置2~8℃保存,定期测定其病毒含量,同时进行25℃、37℃耐老化试验和小鼠免疫原性试验。结果显示,疫苗在2~8℃条件下保存24个月,使用耐热冻干保护剂制备的疫苗病毒含量下降不超过1.0 Log PFU/mL,使用传统冻干保护剂制备的疫苗病毒含量下降超过3.0 Log PFU/mL;25℃常温保存2个月,使用耐热冻干保护剂制备的疫苗病毒含量下降不超过1.0 Log PFU/mL,而使用传统冻干保护剂制备的疫苗病毒含量下降约2.0 Log PFU/mL;37℃保存10 d,使用耐热冻干保护剂制备的疫苗病毒含量下降不超过1.5 Log PFU/mL,使用传统冻干保护剂制备的疫苗下降超过1.5 Log PFU/mL。使用耐热冻干保护剂制备的活疫苗和使用传统冻干保护剂制备的活疫苗对小鼠的免疫原性相当,半数有效剂量分别为10^(-3.12)/0.1 mL和10^(-3.11)/0.1 mL。试验结果表明,耐热冻干保护剂对病毒的保护效力明显优于传统冻干保护剂,具有良好的热稳定性,使用耐热冻干保护剂制备的活疫苗具有良好的免疫原性。
In order to improve the stability of porcine Japanese encephalitis virus live vaccine and facilitate its preservation and transportation,this study prepared live vaccine by lyophilizing and mixing heat-resistant lyophilized protectant and conventional lyophilized protectant,respectively,stored the vaccine at 2-8℃,measured the virus content regularly,tested the aging resistance at 25℃and 37℃,and carried out immunogenicity test in mice.The results showed that when the vaccine was stored at 2-8℃for 24 months,the virus content of the vaccine with heat-resistant lyophilized protectant decreased less than 1.0 Log PFU/mL,while that of the conventional vaccine decreased more than 3.0 Log PFU/mL;when the vaccine was stored at 25℃for 2 months,the virus content of the vaccine with heat-resistant lyophilized protectant decreased less than 1.0 Log PFU/mL,while that of the conventional vaccine decreased about 2.0 Log PFU/mL;when the vaccine was stored at 37℃for 10 days,the virus content of the vaccine with heat-resistant lyophilized protectant decreased no more than 1.5 Log PFU/mL,while that of the conventional vaccine decreased more than 1.5 Log PFU/mL.The immunogenicity of live vaccine prepared with heat-resistant lyophilized protectants was similar to that of live vaccine prepared with conventional lyophilized protectants,with half effective doses of 10^(-3.12)/0.1 mL and 10^(-3.11)/0.1 mL,respectively.Thus,the heat-resistant lyophilized protectant displayed better protective effect on virus than conventional lyophilized protectant,and had good thermal stability;and the preparation of live vaccine with heat-resistant lyophilized protectant maintained good immunogenicity.
作者
韩伟
刘艳彬
蒋利
潘春刚
周建民
HAN Wei;LIU Yan-bin;JIANG Li;PAN Chun-gang;ZHOU Jian-min(China Animal Husbandry Industry Co.Ltd.,Beijing 100070,China;Key Laboratory of Biological Products and Chemical Drugs for Animals,Ministry of Agriculture and Rural Affairs,Beijing 100095,China;Technology Research Center for Beijing Veterinary Peptide Vaccine Design and Preparation,Beijing 100095,China)
出处
《中国兽医杂志》
CAS
北大核心
2022年第7期72-77,共6页
Chinese Journal of Veterinary Medicine
关键词
乙型脑炎病毒(JEV)
疫苗滴度
冻干
耐热冻干保护剂
活疫苗
Japanese encephalitis virus(JEV)
vaccine titer
freeze-dry
heat-resistant lyophilized stabilizer
live vaccine
