摘要
在海南省昌江县棋子湾生长的木麻黄(Casuarina equisetifolia)上发现一种新的灵芝茎腐病(Ganoderma sp.)。通过组织分离法从病原菌担子果中分离出代表菌株HNCJ201801001。对该菌株进行致病性测定、担子果诱导、形态学鉴定,构建rDNA-ITS、LSU系统发育树分析,完成生物学特性测定。依据形态学与分子检测结果,确定海南省昌江县棋子湾木麻黄茎腐病的病原菌为狭长孢灵芝(Ganoderma boninense)。对其进行生物学特性测定,结果表明,该菌的最适温度为28℃,最适pH为5.0,黑暗条件更有利于菌丝生长,在PDA和PSA培养基上生长较好。
A new stem rot caused by Ganoderma sp. was found on Casuarina equisetifolia growing in Qizi Bay, Changjiang County, Hainan Province. A representative strain HNCJ201801001 was isolated from basidiocarps of the pathogen by tissue isolation method. The pathogenicity determination, basidiocarp induction and morphological identification of the strain were carried out, a phylogenetic tree using rDNA-ITS and LSU genes was constructed for analysis of the strain, and the biological characteristics of the strain were determined. According to the results of morphological and molecular detection, the pathogen of C. equisetifolia stem rot in Qizi Bay, Changjiang County, Hainan Province was identified as Ganoderma boninense. The biological characteristics of G. boninense were determined and the results showed that the growth of G.boninense was optimum when cultured at temperature 28 ℃, pH5.0 in dark. Dark condition was more conducive to mycelial growth of G. boninense, and G. boninense grew better on PDA and PSA mediums than on the other mediums.
作者
朱清亮
李增平
张宇
ZHU Qingliang;LI Zengping;ZHANG Yu(School of Plant Protection,Hainan University,Haikou,Hainan 570228,China)
出处
《热带生物学报》
2022年第5期524-531,共8页
Journal of Tropical Biology
基金
国家天然橡胶产业技术体系项目(CARS-34-BC1)
海南大学名师工作室项目(hdms202014)。
关键词
木麻黄
茎腐病
狭长孢灵芝
生物学特性
Casuarina equisetifolia
stem rot
Ganoderma boninense
biological characteristics
