摘要
背景高强度间歇训练(HIIT)作为心脏康复的运动方案之一,对于心肌梗死的作用还存在争议,且作用机制尚不明确。目的探讨HIIT对心肌梗死大鼠心脏结构和功能的影响。方法于2020年9月至2021年10月,随机选取3月龄雄性SD大鼠共78只,最终纳入研究56只。随机选取14只为假手术组(Sham组),其余大鼠制备急性心肌梗死(AMI)模型后随机分为心梗静息组(MI-SED组,n=14)、心梗HIIT组(MI-HIIT组,n=14),心梗中等强度持续训练(MICT)组(MI-MICT组,n=14)。Sham组与MI-SED组不进行训练,MI-HIIT组高强度与中等强度训练交替进行,MI-MICT组进行中等强度持续训练。AMI大鼠建模1周后MI-HIIT、MI-MICT组开始进行训练,训练4周后,四组分别随机取7只大鼠进行心脏超声检测,称量体质量(BW)、心脏质量(HW),计算心脏质量指数(HMI),检测血清肿瘤坏死因子α(TNF-α)水平,并取心脏组织进行HE染色、Masson染色及免疫组化分析;训练8周后对四组剩余7只大鼠采集数据和样本进行相同试验处理。结果训练4周后MI-HIIT组标准化HMI高于Sham组、MI-SED组和MI-MICT组(P<0.05);训练8周后MI-HIIT组最终BW低于Sham组和MI-SED组,HW高于Sham组、MI-SED组和MI-MICT组,标准化HMI高于Sham组和MI-MICT组(P<0.05)。训练4周后,MI-HIIT组和MI-MICT组射血分数(EF)、缩短分数(FS)、左心室收缩末期后壁厚度(LVPWs)低于Sham组,高于MISED组(P<0.05);MI-HIIT组和MI-MICT组左心室收缩末期内径(LVDs)低于MI-SED组(P<0.05)。训练8周后,MI-HIIT组EF、LVDs、左心室舒张末期内径(LVDd)、左心室收缩末期前壁厚度(LVAWs)高于Sham组和MI-SED组,FS、LVPWs高于MI-SED组(P<0.05)。HE染色显示,MI-HIIT组训练8周大鼠的心脏组织炎性病变较训练4周大鼠减轻,心肌细胞排列更紧密。Masson染色结果显示训练8周后MI-HIIT组、MI-MICT组心肌组织胶原纤维占比低于MI-SED组。免疫组化结果显示训练4、8周后MI-HIIT组、MI-MICT组心肌组织血管新生数�
Background As one exercise program of cardiac rehabilitation,the role of high-intensity interval training(HIIT)in improving myocardial infarction is still controversial,and the mechanism is unclear.Objective To investigate the effect of HIIT on improving the cardiac structure and function in a rat model of myocardial infarction.Methods An experiment was conducted from September 2020 to October 2021.From a random sample of 783-month-old male SD rats,56 were eventually included,and 14 of them were randomly selected as sham-surgery group(Sham group),and the remaining 42 rats were equally randomized into MI-sedentary group(MI-SED group),MI-HIIT group(MI-HIIT group),and MImedium-intensity continuous training(MI-MICT group)after being used for preparing a model of acute myocardial infarction(AMI).Sham group and MI-SED group were not trained,MI-HIIT group received high-intensity and medium-intensity training alternately,MI-MICT group received medium-intensity continuous training.After 1 week of AMI modeling,MI-HIIT and MI-MICT groups received 8 weeks of training.At the end of 4 weeks of training,7 rats in each of the 4 groups were randomly selected for detecting cardiac ultrasound,and weighing body weight,then were sacrificed,and their heart weight and serum tumor necrosis factor(TNF-α)were measured,cardiac mass index was calculated,and heart tissues were measured using H&E staining,Masson's Trichrome staining and immunohistochemical staining.At the end of 8 weeks of training,the same operation was performed on the remaining 7 rats in each of the groups.Results After 4 weeks of training,the standardized cardiac mass index of MI-HIIT group was higher than that of each of the other three groups(P<0.05).The sham group had higher ejection fraction(EF),fractional shortening(FS)and left ventricular end-systolic posterior wall thickness(LVPWs)than MI-HIIT and MI-MICT groups(P<0.05).MI-SED group had lower EF,FS,and LVPWs,and higher left ventricular end-systolic diameter(LVESD)than MI-HIIT and MI-MICT groups(P<0.05).After 8 wee
作者
贾思琦
王迪
鲁燕
贾永平
JIA Siqi;WANG Di;LU Yan;JIA Yongping(The First Clinical Medical College,Shanxi Medical University,Taiyuan 030000,China;Department of Cardiovascular Medicine,First Hospital of Shanxi Medical University,Taiyuan 030000,China)
出处
《中国全科医学》
CAS
北大核心
2022年第33期4159-4167,共9页
Chinese General Practice
基金
山西省重点研发计划项目(201803D31092,201903D321180)。
关键词
心肌梗死
大鼠
运动
炎症
心室重构
心脏康复
高强度间歇训练
Myocardial infarction
Rats
Exercise
Inflammation
Ventricular remodeling
Cardiac rehabilitation
High-intensity interval training
