摘要
目的探究活血消异方对子宫内膜异位大鼠内膜容受性及CXCL12/CXCR4信号通路的作用机制。方法将50只SPF级SD雌性大鼠按照数字随机法分为假手术组、模型组(子宫内膜异位症大鼠模型组)、中药组(子宫内膜异位症大鼠模型组给予活血消异方干预治疗组)、西药组(子宫内膜异位症大鼠模型组给予醋酸亮丙瑞林干预组)和拮抗剂组(子宫内膜异位症大鼠模型组给予AMD3100干预组),每组10只(只在检测CXCL12和LXCR4蛋白表达中使用拮抗剂组干预)。ELISA检测4组大鼠性指标E_(2)、P水平;HE染色比较4组大鼠子宫内膜组织病理学变化;LIF、αvβ3、CXCL12和CXCR4蛋白(Western blot检测);CXCL12和CXCR4 mRNA表达(RT-PCR)。结果与假手术组相比,模型组大鼠血清中性激素指标E_(2)、P含量均显著降低(P<0.05);与模型组相比,西药组和中药组血清中性激素指标E_(2)、P含量均不同程度升高,且中药组E_(2)、P含量升高的较为显著(P<0.05)。模型组大鼠子宫内膜中LIF、αvβ3蛋白表达明显低于假手术组(P<0.05);干预后的西药组和中药组中的LIF、αvβ3蛋白表达明显升高,且中药组升高较为显著(P<0.05)。模型组CXCL12和CXCR4蛋白和相对表达量较假手术组明显升高(P<0.05);干预后的西药组、中药组和拮抗组大鼠子宫内膜组织中CXCL12和CXCR4蛋白和相对表达均明显低于模型组,且中药组和拮抗组大鼠CXCL12和CXCR4蛋白和相对表达较西药组降低的更为显著(P<0.05)。结论活血消异方对子宫内膜异位症大鼠子宫内膜容受性进行有效的改善,同时可抑制子宫内膜异位症大鼠CXCL12、CXCR4表达。
Objective To explore the mechanism of Huoxue Xiaoyi Recipe on endometrial receptivity and CXCL12/CXCR4 signaling pathway in rats with endometriosis.Methods The rats were divided into sham operation group,model group(endometriosis rat model group),traditional Chinese medicine group(endometriosis rat model group was given Huoxue Xiaoyi prescription intervention treatment group)and western medicine group(endometriosis rat model group was given leproteroline acetate intervention group)according to digital random method,with 10 rats in each group.The levels of E2 and P in serum were detected by ELISA,the histopathological changes of endometrium were detected by HE staining,and the levels of LIF and P in endometrium were detected by ELISAαvβ3 The protein expressions of cxcl3,LIF,αvβ3.CXCL12 and CXCR4 protein expression were detected by Western blot.CXCL12 and CXCR4 mRNA expression were detected by RT-PCR.Results The levels of E2 and P in the model group were significantly lower than those in the sham operation group(P<0.05).Compared with the model group,the levels of E2 and P in western medicine group and traditional Chinese medicine group were increased in varying degrees,and the levels of E2 and P in traditional Chinese medicine group were significantly increased(P<0.05).In the model group,the expression of LIF andαvβ3 protein expression was significantly lower than that in sham operation group(P<0.05).After the intervention,LIF in the western medicine group and the traditional Chinese medicine groupαvβ3 protein expression increased significantly,and Chinese medicine group increased more significantly(P<0.05).The protein and relative expression of CXCL12 and CXCR4 in endometrium of model group were significantly higher than those of sham operation group(P<0.05).After intervention,the protein and relative expression of CXCL12 and CXCR4 in endometrium of Western medicine group and traditional Chinese medicine group were significantly lower than those of model group,and the protein and relative expression of CXC
作者
赵秀萍
郝秀芳
朱秀娟
ZHAO Xiuping;HAO Xiufang;ZHU Xiujuan(The Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《西部医学》
2022年第9期1296-1301,共6页
Medical Journal of West China
基金
北京中医药大学中央高校基本科研项目(2019-JYB-JS-166)。
