摘要
【目的】探究外源大麻二酚(CBD)对重金属胁迫下烟草种子萌发和幼苗生长的影响。【方法】以K326和白肋烟鄂烟1号种子为材料,研究不同质量浓度CBD(0、1、5、25 mg/L)对Cr(8 mg/L)和Cd(30 mg/L)胁迫下烟草种子萌发和幼苗生长的影响。【结果】无重金属胁迫时,施用CBD可轻微抑制K326种子的萌发,其发芽率最多降低2.78%,但能提高幼苗素质;施用25 mg/L CBD可显著提高鄂烟1号种子的发芽率和活力指数,分别显著提高5.79%和47.32%。重金属胁迫下,施用5和25 mg/L CBD分别对Cr胁迫下K326和鄂烟1号种子萌发的缓解效果最佳,K326和鄂烟1号的种子活力指数分别比单独Cr处理显著提高49.18%和30.87%,死亡率分别下降67.73%和76.04%。5 mg/L CBD对Cd胁迫的缓解效果最好,烟草种子发芽指数和干质量分别比单独Cd处理提高8.11%和79.24%(K326)、25.81%和82.15%(鄂烟1号)。【结论】CBD能有效缓解Cr和Cd胁迫对烟草种子萌发的不利影响,促进幼苗生长,但CBD的适宜质量浓度因重金属的种类、胁迫程度和植株品种的不同而不同。
[Purpose]To explore the effect of exogenous cannabidiol(CBD)on tobacco seed germination and seedling growth under heavy metal stress.[Methods]K326 and burley tobacco Eyan No.1 seeds were used to study the effects of different mass concentrations of CBD(0,1,5,25 mg/L)on the indexes of tobacco seed germination and seedling growth under Cr(8 mg/L)and Cd(30 mg/L)stress.[Results]The addition of CBD could slightly inhibit the germination of K326 seeds,reduced the germination rate by 2.78%at most,but improve the quality of seedlings in the absence of heavy metals.The germination rate and vigor index of Eyan No.1 seeds added with 25 mg/L CBD were significantly increased by 5.79%and 47.32%,respectively.Under heavy metal stress,5 and 25 mg/L CBD had the best alleviation on the seed germination of K326 and Eyan No.1 under Cr stress.The seed vigor index of K326 and Eyan No.1 was significantly increased by 49.18%and 30.87%compared with Cr treatment alone,and the mortality was reduced by 67.73%and 76.04%,re-spectively.5 mg/L CBD had the best alleviation on Cd stress.The germination index and dry weight of tobacco seeds were increased by 8.11%and 79.24%(K326),25.81%and 82.15%(Eyan No.1),re-spectively compared with Cd treatment alone.[Conclusion]CBD could effectively alleviate the toxic effects of Cr and Cd on tobacco seed germination,and promote seedling growth.However,the appropriate mass concentration of CBD should be selected according to the type of heavy metals,stress degree and plant varieties.
作者
管娇
杨嘉玲
邹红旭
翁玉仙
樊兴荣
王丽华
保志娟
GUAN Jiao;YANG Jialing;ZOU Hongxu;WENG Yuxian;FAN Xingrong;WANG Lihua;BAO Zhijuan(College of Tobacco Science,Yunnan Agricultural University,Kunming 650201,China)
出处
《云南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第5期835-841,共7页
Journal of Yunnan Agricultural University:Natural Science
基金
云南省科技计划农业联合面上项目(2017FG001-063)
云南省教育厅科学研究基金研究生项目(2020Y161)。
关键词
大麻二酚
烟草
重金属胁迫
种子萌发
缓解作用
cannabidiol
tobacco
heavy metals stress
seed germination
alleviation
