摘要
为探究人工鱼礁投放对底泥细菌群落结构和功能的影响,于2018年9月对2014年投礁人工鱼礁区、2018年6月新投礁区和对照区进行表层沉积物细菌16S rRNA基因测序和环境因子测定。利用高通量测序技术对细菌16S rRNA基因V4~V5区进行测序,并结合环境因子分析细菌群落的结构变化和影响因子。结果表明,投礁2个月后,新投礁区的细菌群落结构逐渐与鱼礁区趋于一致,鱼礁区和新投礁区细菌物种数和多样性指数均高于对照区,且差异显著(P<0.05)。从门类上看,变形菌门是渤海湾沉积物中丰度最高的菌种,对照区变形菌门和拟杆菌门丰度低于其他2个区域,厚壁菌门和蓝细菌丰度显著高于其他2个区域。从种类上看,对照区细菌种类明显多于其他2个区域,鱼礁投放使细菌种类发生了聚集,硫氧化菌丰度显著降低,海洋伍斯菌和脱硫细菌丰度显著升高。环境因子检测表明,与本底值和对照区相比,人工鱼礁的投放使海域底泥的粒径变小,夏季溶解氧和硫化物含量降低。功能基因和影响因子相关分析表明,鱼礁区底泥细菌的硫还原反应影响了礁区的环境,同时礁区环境的改变也影响了菌群的结构。
In order to study the impact of artificial reef release on sediment bacteria, sediment bacteria detection and environmental factor determination were conducted in artificial reef area built in 2014, newly built reef area in 2018 and control area by high-throughout squencing technology in September 2018. The results showed that after two months of reef-release, the bacterial community structure in the reef release area tended to be gradually consistent with that in the newly built reef release area, and the diversity index and species number in the control area were lower than that in the reef-throwing area, with significant differences(P<0.05). Proteobacteria were the most abundant strains in Bohai Bay. The abundance of Proteobacteria and Bacteroidetes in the control area was lower than that in the other two regions. The abundance of Firmicutes and cyanobacteria in the control area was significantly higher than that in the other two regions. In terms of species, the bacterial species in the control area were significantly more than those in the other two regions, and the abundance of Sulfurovum was significantly higher than that in the other two regions. However, the abundance of Woeseia and desulfurization bacteria in the other two regions was significantly higher than that in the control area. Compared with the background value and the control area, the particle size of the sediment and the sulfide content were decreased in the artificial reef, and dissolved oxygen concentration were reduced in summer. Correlation analysis of functional genes and influencing factors showed that the sulfur reduction reaction of the reef sediment bacteria affected the reef environment, and the change of the reef environment also affected the structure of the bacterial flora.
作者
王宇
徐晓甫
于莹
郭彪
高燕
张雪
张博伦
张晶伟
WANG Yu;XU Xiaofu;YU Ying;GUO Biao;GAO Yan;ZHANG Xue;ZHANG Bolun;ZHANG Jingwei(Tianjin Marine Reach Engineering Technology Center,Department of Fisheries Resources,Tianjin Fisheries Research Institute,Tianjin 300457,China;National Marine Data and Information Service Centre,Marine Information Technology Innovation Center,Ministry of Natural Resources,Tianjin 300171,China)
出处
《水产科学》
CAS
CSCD
北大核心
2022年第5期799-809,共11页
Fisheries Science
基金
天津市科委重大科技专项(18ZXRHSF00270)
天津市农业发展服务中心青年科技创新项目(ZXKJ201915)
自然资源部海洋信息技术创新中心开放基金资助项目(B201801028)。
关键词
天津大神堂海域
人工鱼礁
底泥细菌
群落特征
功能分析
Dashentang sea area in Tianjin
artificial reef
sediment bacterium
community characteristics
function analysis
