摘要
为探讨脱酚棉籽粕(LCSM)替代鱼粉对卵形鲳鲹(Trachinotus ovatus)的肌肉品质、抗氧化能力及相关基因表达的影响,本研究选择体质量为28.42±0.02 g的卵形鲳鲹,分别利用LCSM替代0%、20%、40%、60%的鱼粉蛋白,配制了4组饲料(分别记为LCSM0、LCSM20、LCSM40、LCSM60组),饲养卵形鲳鲹6周。结果显示:(1)LCSM20组的肌肉硬度、弹性、内聚性、胶粘性、回复性与LCSM0组的差异无统计学意义(P>0.05),胶着性和咀嚼性显著下降(P<0.05);LCSM40组和LCSM60组的内聚性显著提高(P<0.05),其余质构指标显著下降(P<0.05)。(2)LCSM20、LCSM40、LCSM60组的pH和蒸煮损失率与LCSM0组相比差异有统计学意义(P<0.05),肌肉滴水损失率的差异无统计学意义(P>0.05)。(3)LCSM20组和LCSM40组的肌肉胶原蛋白(Collagen)的质量分数与LCSM0组相比差异无统计学意义(P>0.05),LCSM60组的显著下降(P<0.05)。(4)LCSM20组的丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)含量与LCSM0组相比差异无统计学意义(P>0.05),超氧化物歧化酶(SOD)含量显著下降(P<0.05);LCSM40组和LCSM60组的MDA含量与LCSM0组相比差异无统计学意义(P>0.05),SOD含量和GSH-Px含量显著下降(P<0.05)。(5)LCSM20组肌肉品质相关基因(Decorin、MSTN、MyoG和CatL)的转录水平与LCSM0组相比差异无统计学意义(P>0.05),COL1α1、CatB基因的转录水平显著上调(P<0.05);LCSM40组和LCSM60组的MSTN、COL1α1基因的转录水平与LCSM0组相比显著上调(P<0.05),LCSM40组的Decorin、MyoG、CatB和CatL基因的转录水平与LCSM0组相比差异无统计学意义(P>0.05),LCSM60组的Decorin基因的转录水平与LCSM0组相比显著下调(P<0.05),MyoG基因的转录水平与LCSM0组相比差异无统计学意义(P>0.05),CatB、CatL基因的转录水平与LCSM0组相比显著上调(P<0.05)。综上所述,以对肌肉品质、抗氧化能力及相关基因表达的影响为评价指标,利用脱酚棉籽粕替代卵形鲳鲹饲料鱼粉的最适比例为20%。
In order to investigate the effect of replacing fish meal with low-phenol cottonseed meal(LCSM) on flesh quality, antioxidant capacity and related gene expression of golden pompano, 4 groups of LCSM-added feeds were prepared to replace 0%(LCSM0), 20%(LCSM20), 40%(LCSM40) and 60%(LCSM60) fish meal protein and golden pompano of 28.42±0.02 g were fed for 6 weeks. The results are as follows. First, there was no statistically significant difference in flesh hardness, elasticity, cohesion, glue viscosity and recovery between LCSM20 group and LCSM0 group(P>0.05), and the adhesion and chewiness of LCSM20 group decreased significantly(P<0.05);the cohesion of LCSM40 group and LCSM60 group increased significantly(P<0.05), and the other texture indexes decreased significantly(P<0.05). Second, there was statistically significant difference in pH and cooking loss rate of LCSM20, LCSM40 and LCSM60 group compared with LCSM0 group(P<0.05), and there was no statistically significant difference in flesh drip loss rate(P>0.05). Third, there was no statistically significant difference in mass fraction of collagen of LCSM20 and LCSM40 group compared with LCSM0 group(P>0.05). Fourth, the content of malondialdehyde(MDA) and the activity of glutathione peroxidase(GSH-Px) in LCSM20 group showed no change compared with LCSM0 group(P>0.05), but the SOD activity was down-regulated in LCSM20 group(P<0.05). There was no statistically significant difference in MDA content between LCSM40 and LCSM60 group compared with LCSM0 group(P>0.05). The SOD activity and GSH-Px content were deceased in LCSM40 and LCSM60 group(P<0.05). Fifth, there was no statistically significant difference in the transcription level of flesh quality-related genes(Decorin, MSTN, MyoG and CatL) in LCSM20 group compared with LCSM0 group(P>0.05). The COL1α1 and CatB gene transcription levels were significantly up-regulated(P<0.05). The transcription levels of MSTN and COL1α1 genes in the LCSM40 and LCSM60 groups were significantly up-regulated compared with LCSM0 group(P<0.
作者
苗玉涛
钟宛清
卢宝鑫
付胜利
宋飞
陈义烘
汪蕾
MIAO Yutao;ZHONG Wanqing;LU Baoxin;FU Shengli;SONG Fei;CHEN Yihong;WANG Lei(School of Life Science/Institute of Modern Aquaculture Science and Engineering/Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture,South China Normal University,Guangzhou 510631,China;Southern Marine Science and Engineering Guangdong Laboratory,Zhuhai 519000,China;Southern Marine Science and Engineering Guangdong Laboratory,Zhanjiang 524025,China)
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2022年第4期65-73,共9页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金项目(31500326)
广州市科技计划项目(202102020234)
南方海洋科学与工程广东省实验室项目(ZJW-2019-06)。
关键词
脱酚棉籽粕
鱼粉
卵形鲳鲹
肌肉品质
low-phenol cottonseed meal
fish meal
golden pompano
flesh quality
