摘要
[目的]揭示巨桉基因组中生长素响应因子(auxin response factor, ARF)基因家族的特征,为深入解析其生物学功能奠定基础。[方法]基于生物信息学分析巨桉中鉴定出17个ARF基因,并对其基因结构特征和表达模式进行综合研究。[结果]巨桉ARF不均匀分布在9条染色体上,二级结构均含有α-螺旋、延伸链、无规卷曲和β-转角。根据系统发生关系分成4个亚族。表达分析表明ARF基因在巨桉的根、茎、叶等各组织中均有表达,但表达量存在差异。[结论]巨桉Eucgr.F04380、Eucgr.D00264和Eucgr.D00588在花中特异性表达,Eucgr.D01764、Eucgr.E00888和Eucgr.K03433在分生组织中特异性表达。
[Objective]This study aims to reveal the characteristics of the auxin response factor(ARF) gene family in the genome of Eucalyptus grandis and lay a foundation for further understanding its biological function. [Method]Based on bioinformatics analysis, 17 ARF genes were identified in Eucalyptus grandis, and their structural characteristics and expression patterns were comprehensively studied. [Result]The ARF of Eucalyptus grandis is unevenly distributed on 9 chromosomes, and the secondary structure contains α-helix, extended strand, random coil and β-turn. According to the phylogenetic relationship, it is divided into four sub-families. Expression analysis showed that the ARF gene is expressed in the roots, stems and leaves of Eucalyptus grandis, but the amount of expression is different. [Conclusion]Eucgr.F04380, Eucgr.D00264, and Eucgr.D00588 are specifically expressed in flowers, and Eucgr.D01764, Eucgr.E00888 and Eucgr.K03433 are specifically expressed in meristems, laying the foundation for further exploration of their functional identification.
作者
何春玲
齐妍
沈超
HE Chun-ling;QI Yan;SHEN Chao(Maoming Agricultural Science and Technology Extension Center,Maoming,Guangdong 525000;School of Biological and Food Engineering,Guangdong University of Petrochemical Technology,Maoming,Guangdong 525000)
出处
《安徽农业科学》
CAS
2022年第17期83-88,共6页
Journal of Anhui Agricultural Sciences
基金
广东石油化工学院人才引进项目(2019rc112)。
关键词
巨桉
ARF
系统进化
表达分析
Eucalyptus grandis
ARF gene
Phylogenic evolution
Expression analysis
