摘要
为调查我国部分地区禽偏肺病毒(a MPV)的感染情况,本研究从福建、四川、广东、江苏4省的8个地区采集鸡、鸭、鹅、鸽的咽喉/泄殖腔双拭子样品共1 920份,利用四重荧光定量RT-PCR方法检测A、B、C、D亚群的aMPV。结果显示,1 920份样品中共检出aMPV阳性样品51份,其中,B亚群39份,C亚群7份,B亚群和C亚群混合感染5份。4个省aMPV的总阳性率为2.67%(51/1 920),各省aMPV阳性率为0.63%~3.96%;场点阳性率为21.21%(7/33),其中批发市场、零售市场、养殖场和屠宰场场点阳性率分别为80%(4/5)、23%(3/13)、0(0/14)和0(0/1)。鸡a MPV阳性率为3.42%(47/1 373),其中B亚群阳性率为2.78%(38/1 373),C亚群阳性率为0.29%(4/1 373),B和C亚群混合感染阳性率为0.36%(5/1 373),未检测到A和D亚群aMPV。鸽B亚群阳性率为2.63%(1/38),未检测到A、C和D亚群aMPV。鸭C亚群阳性率0.64%(3/466)。研究表明,我国鸡、鸭、鸽中存在aMPV感染,鸡为其主要宿主,B亚群aMPV为优势流行亚群,并首次发现国内鸡群存在B、C亚群aMPV的混合感染。本研究不仅丰富了a MPV的流行病学资料,也为其疫苗研制提供数据参考。
In order to investigate the infection of avian metapneumovirus in parts of China, 1920 throat/cloacal swab samples of chickens, ducks, geese and pigeons were collected from 8 areas of Fujian, Sichuan, Guangdong and Jiangsu provinces.Quadruple fluorescence quantitative RT-PCR was used to detect avian metapneumovirus subgroups A, B, C and D. The results showed that 51 positive samples of avian metapneumovirus were detected in 1920 samples, including 39 samples of subgroup B, 7samples of subgroup C, and 5 samples of mixed infection of subgroup B and subgroup C. The individual positive rate of avian metapneumovirus in four provinces was 2.67%(51/1920), and the individual positive rate in each province was 0.63%-3.96%, and the positive rate in sites was 21.21%(7/33), the positive rates of wholesale markets, reail markets, farms and slaughterhouses were80%(4/5), 23%(3/13), 0(0/14) and 0(0/1) respectively. The positive rate of individual chicken was 3.42%(47/1373), of which the positive rate of subgroup B was 2.78%(38/1373), the positive rate of subgroup C was 0.29%(4/1373), and the positive rate of mixed infection of subgroup B and C was 0.36%(5/1373). Subgroups A and D were not detected. The positive rate of pigeon subgroup B was 2.63%(1/38), and no subgroups A, C and D were detected. The positive rate of duck C subgroup was 0.64%(3/466). The results showed that there was aMPV infection in chickens, ducks and pigeons in China, and chickens are the main epidemic hosts. Subgroup B avian metapneumovirus is the dominant subgroup. Mixed infection of subgroup B and C avian metapneumovirus was found for the first time in domestic chicken flocks. This study not only enriches the epidemiological data of aMPV, but also provides data for its vaccine development.
作者
肖志宇
王素春
赵成龙
黄禹丰
陈海燕
崔成都
孙福亮
王楷宬
XIAO Zhi-yu;WANG Su-chun;ZHAO Cheng-long;HUANG Yu-feng;CHEN Hai-yan;CUI Cheng-du;SUN Fu-liang;WANG Kai-cheng(China Animal Health and Epidemiology Center,Qingdao 266032,China;Yanbian University,Yanji 133000,China;College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471023,China;Qingdao Yebio Bio-Engineer Co.,Ltd.,State Key Laboratory of Genetically Engineered Veterinary Vaccine,Qingdao 266114,China)
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2022年第7期712-717,共6页
Chinese Journal of Preventive Veterinary Medicine
基金
青岛市科技服务计划(18-6-3-1-jch)。
关键词
禽偏肺病毒
亚群
流行病学调查
感染情况
avian metapneumovirus
subgroup
epidemiological investigation
infection
