摘要
为了得到具有耐盐碱及促生特性的菌株,采用梯度稀释涂布法从黑果枸杞(Lycium ruthenicum)根际土壤中分离得到110株根际微生物,通过革兰氏染色和平板划线法观察菌落形态和生长特性,选择37株进行16S rRNA基因序列比对,结果显示37株菌株分别属于9个属,其中芽孢杆菌(Bacillus)为优势属.在NaCl_(2)质量分数为2%~25%和pH为5~12的培养基中对上述菌株进行筛选及除盐、除碱特性研究,并采用固氮、溶磷、产吲哚乙酸(IAA)和产铁载体的定性、定量法分析促生特性.结果显示:10株根际细菌在NaCl质量分数为2%~20%和pH为5~11的培养基中均能生长,在36 h时除盐、除碱效果最好,其中菌株HQA8的除盐、除碱效率分别达75%和46%.上述10株耐盐碱根际细菌在阿须贝无氮培养基中也能生长,溶磷量为28.20~52.63 mg/L.其中菌株HQA2在60 h时产IAA达54.21 mg/L,且产铁载体能力也最强,菌落周围产生的黄色晕圈直径为37.25 mm.综上,筛选获得的10株黑果枸杞根际耐盐碱细菌可为开发生物菌肥和改良土壤极端环境提供优良菌株.
In order to obtain strains with saline-alkali tolerance and growth promoting characteristics,110 rhizosphere microorganisms were isolated from the rhizosphere soil of Lycium ruthenicum by gradient dilution coating method.The colony morphology and growth characteristics were observed by Gram staining and plate scribe method.37 strains were selected for 16S rRNA gene sequence comparison.The results showed that 37 strains belonged to 9 genera,among which Bacillus was the dominant genus.The above strains were screened in the medium with NaCl mass fraction of 2%-25%and pH of 5-12,and the characteristics of salt and alkali removal were studied.The growth promoting characteristics were studied by qualitative and quantitative methods of nitrogen fixation,phosphorus dissolution,indole acetic acid(IAA)production and iron carrier production.The results showed that 10 rhizosphere bacteria could grow in the medium with NaCl mass fraction of 2%-20%and pH of 5-11.The removal efficiency of salt and alkali was the best at 36 h,and the salt and alkali removal rates of strain HQA8 were 75%and 46%,respectively.The above 10 strains of salt and alkali tolerant rhizosphere bacteria could also grow in Ashby nitrogen-free medium.The mass concentration of dissolved phosphorus was 28.20-52.63 mg/L.The IAA production capacity of strain HQA2 reached 54.21 mg/L and the iron carrier production capacity performed the best,and the diameter of surrounding yellow circle was 37.25 mm.In conclusion,the 10 strains of salt and alkali tolerant rhizosphere bacteria of L.ruthenicum can provide excellent strains for the development of biological bacterial fertilizer and the improvement of soil extreme environment.
作者
热孜亚·吐尔逊
郑勇
阿丽亚·外力
特列吾·哈杰提
黄晶晶
玛依拉·买苏提
依丽米努尔·艾力
艾斯凯尔·玉苏普
旭格拉·哈布丁
TURSUN Raziya;ZHENG Yong;WALI Aliya;HAJIETI Teliewu;HUANG Jingjing;MAISUTI Mayira;ALI Elminur;YUSUP Askar;HABDEN Xugela(Xinjiang Key Laboratory of Plant Stress Biology in Arid Land,Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,Xinjiang Key Laboratory of Special Environment Biodiversity Application and Regulation,College of Life Science,Institute of Desert Algae,Xinjiang Normal University,Xinjiang Urumqi 830054)
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第5期862-871,I0001,共11页
Journal of Xiamen University：Natural Science
基金
自治区重点研发计划(2016B03041-2)
新疆干旱区植物逆境生物学实验室项目(XJNUSYS112018A01)。
关键词
黑果枸杞
根际细菌
耐盐碱
除盐
除碱
溶磷
吲哚乙酸
铁载体
Lycium ruthenicum
rhizosphere bacteria
saline-alkali tolerance
desalting
dealkalination
dissolved phosphorus
indole acetic acid
iron carrier
