摘要
为了探究胰岛素样生长因子1(insulin like growth factor 1,IGF1)基因对家禽生长性状的影响,试验利用PCR扩增结合直接测序的方法,对黔东南小香鸡单核苷酸多态性(SNP)进行筛选,并对SNP位点所对应的基因型遗传学进行分析,同时运用SSPS 18.0软件中一般线性模型对基因型与体重、体尺指标进行关联性分析。结果表明:在试验群体中发现IGF1基因第3外显子62 bp处存在1个SNP位点,该位点属于同义突变,并未引起编码氨基酸改变,GA基因型为优势基因型,G等位基因为优势等位基因,该基因座满足Hardy-Weinberg平衡(P>0.05);该SNP位点与黔东南小香鸡体重、体尺性状不存在显著性关联。说明IGF1基因第3外显子62 bp处发生G>A突变,虽然其不影响黔东南小香鸡的生长性状,但可以考虑作为黔东南小香鸡分子遗传标记辅助选育参考位点之一。
In order to explore the effect of insulin-like growth factor 1(IGF1)gene on the growth traits of poultry,the experiment used the method of PCR amplification combined with direct sequencing to screen the single nucleotide polymorphism(SNP)of Qiandongnan Xiaoxiang chickens,and analyzed the genotype genetics corresponding to the SNP locus.At the same time,the GLM model in the SSPS 18.0 software was used to analyze the correlation between genotype and body weight and body size.The results showed that there was a SNP site at 62 bp of exon 3 of IGF1 gene in the experimental population,which was a synonymous mutation and did not cause changes in the encoded amino acid.The GA genotype was the dominant genotype;allele G was the dominant allele;and this locus was in accordance with the Hardy-Weinberg equilibrium(P>0.05).There was no significant association between this SNP locus and the growth traits of Qiandongnan Xiaoxiang chickens.The results suggested that the G/A mutation occured at 62 bp in exon 3 of IGF1 gene.Although it did not affect the growth traits of Qiandongnan Xiaoxiang chickens,it could be considered as one of the reference sites for molecular genetic marker-assisted breeding of Qiandongnan Xiaoxiang chickens.
作者
周迪
赵忠海
李建伟
李俊
杨蓉
任丽群
龙清孟
袁勇
严兴烨
ZHOU Di;ZHAO Zhonghai;LI Jianwei;LI Jun;YANG Rong;REN Liqun;LONG Qingmeng;YUAN Yong;YAN Xingye(Guizhou Province Testing Center for Breeding Livestock and Poultry Germplasm,Guiyang 550018,China;Zunyi City Animal Husbandry and Fishery Industry station,Zunyi 563000,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2022年第15期120-122,130,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
贵州省地方家禽产业联合攻关项目(黔财农〔2020〕175号)
中央引导地方发展专项(黔科中央地〔2018〕4015)
贵州省生态家禽产业技术体系建设项目。
关键词
IGF1基因
SNP
体重
体尺性状
关联分析
黔东南小香鸡
IGF1 gene
polymorphism
weight
body size traits
correlation analysis
Qiandongnan Xiaoxiang chickens
