摘要
目的:探究凝血酶激活纤溶抑制物(TAFI)基因多态性、性激素与妊娠早期先兆流产结局关系。方法收集本院2018年-2020年间收治的先兆流产113例(先兆组)与正常早孕96例妇(对照组)临床资料,采用等位基因特异性聚合酶链反应(AS-PCR)检测TAFI1040C/T位点基因分型,化学发光法检测性激素水平。根据先兆组妊娠结局分为难免流产组与继续妊娠组,比较各特征孕妇TAFI1040C/T位点基因分型、性激素水平,分析TAFI1040C/T位点基因型与先兆组妊娠结局及其性激素水平关系。结果:先兆组113例发生难免流产41例,余72例继续妊娠。先兆组血绒毛膜促性腺激素(β-HCG)、雌二醇(E_(2))以及黄体生成激素(LH)水平均低于对照组,且难免流产者均低于继续妊娠者(均P<0.05)。先兆组及对照组TAFI1040C/T基因分布符合H-W平衡,先兆组TT型基因频率高于对照组、CC型基因频率低于对照组,两组TAFI1040基因型分布有差异(P<0.05)。先兆组中难免流产者TT型基因频率高于继续妊娠者,CC型及CT型基因频率低于继续妊娠者,CC型、CT型及TT型基因型患者血β-HCG、P、E_(2)水平依次下降(均P<0.05)。结论:TAFI1040C/T等位基因T可能是先兆流产及不良妊娠结局的危险基因,可能通过影响β-HCG、P、E_(2)水平影响患者妊娠结局。
Objective:To investigate the correlation of thrombin activatable fibrinolysis inhibitor(TAFI)gene polymorphism and serum sex hormone level of the women with threatened abortion during the first trimester of pregnancy and their pregnancy outcomes.Methods:The clinical data of 113 women with threatened abortion during the first trimester of pregnancy(in study group)and another 96 normal pregnancy women(in control group)from 2018 to 2020 were collected.Genotyping of TAFI1040C/T polymorphisms were detected by allele-specific polymerase chain reaction(AS-PCR).The levels of serum sex hormones were detected by chemiluminescence immunoassay.The women in the study group were further divided in group A(women with inevitable abortion)and group B(women with continued pregnancy).The genotypes of TAFI1040C/T locus and the sex hormone levels were compared among the pregnant women with different characteristics.The correlation between TAFI1040C/T locus genotype of the women in the study group and their pregnancy outcomes and sex hormone levels was analyzed.Results:In the study group,there were 41 women with inevitable abortion and 72 women with continued pregnancy.The levels of serum chorionic gonadotropin(β-hCG),estradiol(E_(2)),and luteinizing hormone(LH)of the women in the study group were significantly lower than those of the women in the control group,and which of the women in group A were significantly lower than those of the women in group B(all P<0.05).The distributions of TAFI1040C/T gene of the women in the study group and the control group were in line with H-W balance.The frequency of TT gene of the women in the study group was significantly higher than that of the women in the control group,the frequency of CC gene of the women in the study group was significantly lower than that of the women in the control group,and there was significant difference in the distribution of TAFI1040C/T gene of the women between the two groups(P<0.05).In the study group,the frequency of TT gene of the women with inevitable abortion was
作者
董晓娟
何婵凤
王妍
DONG Xiaojuan;HE Chanfeng;WANG Yan(Jinshan Branch of Shanghai Sixth People's Hospital,Shanghai,201599)
出处
《中国计划生育学杂志》
2022年第9期2072-2075,2080,共5页
Chinese Journal of Family Planning
关键词
先兆流产
基因多态性
凝血酶激活纤溶抑制物
性激素
妊娠结局
Threatened abortion
Gene polymorphism
Thrombin activatable fibrinolysis inhibitor
Sex hormone
Pregnancy outcomes
