摘要
目的探讨脂联素参与七氟醚预处理后心肌缺血再灌注(MIR)小鼠的认知功能保护作用的机制。方法将野生健康成年SPF级雄性C57BL/6小鼠随机分为sham组、MIR-Control组、MIR-SevoPre组,每组10只。将健康成年SPF级雄性脂联素基因敲除(APN KO)小鼠随机分为KO-sham组、KO-MIR-Control组、KOMIR-Sevopre组、KO-MIR-Sevopre-AdipoRon组,每组10只。检测3组C57BL/6小鼠在模型复制或对照处理后12 h血浆的高、中和低分子量脂联素浓度;使用Morris水迷宫实验检测3组C57BL/6小鼠在模型复制或对照处理后24 h的游泳速率和潜伏期;检测4组APN KO小鼠在模型复制或对照处理后第1天、第2天、第4天的潜伏期。结果3组C57BL/6小鼠模型复制后第1天、第2天和第4天的游泳速率比较,采用重复测量设计的方差分析,结果:①不同时间点的小鼠游泳速率无差异(P>0.05);②3组小鼠游泳速率无差异(P>0.05);③3组小鼠游泳速率的变化趋势无差异(P>0.05)。3组C57BL/6小鼠模型复制后第1天、第2天和第4天的潜伏期比较,采用重复测量设计的方差分析,结果:①不同时间点的小鼠潜伏期有差异(P<0.05);②3组小鼠的潜伏期有差异(P<0.05),与sham组比较,MIR-Control组各时间点潜伏期延长(P<0.05),与MIR-Control组比较,MIR-Sevopre组小鼠各时间点潜伏期缩短(P<0.05);③3组小鼠的潜伏期变化趋势有差异(P<0.05)。3组C57BL/6小鼠血浆脂联素水平比较,总脂联素、高分子量脂联素、中分子量脂联素差异有统计学意义(P<0.05),低分子量脂联素差异无统计学意义(P>0.05)。Sham组小鼠的潜伏期与血浆脂联素水平呈负相关(r=-0.480,P=0.033),MIR-Control组小鼠的潜伏期与血浆脂联素水平呈负相关(r=-0.300,P=0.040),MIR-Sevopre组小鼠的潜伏期与血浆脂联素水平呈负相关(r=-0.730,P=0.019)。4组APN KO小鼠模型复制后第1天、第2天和第4天的潜伏期比较,采用重复测量设计的方差分析,结果:①不同时间点小鼠潜
Objective To investigate whether Adiponectin is involved in the protective effect of myocardial ischemia reperfusion(MIR)in mice pretreated with Sevoflurane.Methods Wild C57BL/6 male mice were randomly divided into three groups,each group had 10 mice:sham(sham Operation Group),MIR-control(model group),and MIR-Sevopre(pretreatment group).Male adiponectin knockout C57BL/6(APN KO)mice were randomly divided into 4 groups,each group consisted of 10 Mice:KO-sham(knock-gene sham Group),KO-MIRControl(knock-gene-Model-Group),KO-MIR-Sevopre(knock-gene-model-pretreatment-Group),and KO-Sevopreadiporon-MIR(knock-gene-pretreatment-drug-group).The concentration of high,middle,and low molecular weight adiponectin in plasma of C57BL/6 mice in three groups was detected at 12h after modeling or control treatment.The swimming rate and swimming latency of C57BL/6 mice in three groups were detected at 24h after modeling or control treatment by Morris water maze.The incubation period of mice in APN KO four groups was detected on the 1st,2nd,and 4th day after modeling or control treatment.Results The swimming rates of C57BL/6 mice in the three groups were compared on the 1st,2nd,and 4th day after model replication.The repeated measurement design was used to analyzed the results,which showed:(1)There was no difference in the swimming rates of mice at different time points(P>0.05);(2)There was no difference in swimming speed among the three groups(P>0.05);(3)There was no difference in the trend of swimming speed among the three groups(P>0.05).The latency of C57BL/6 mice in the three groups was compared on the 1st,2nd,and 4th days after model replication.The repeated measurement design was used to analyzed the results,which showed:(1)there were differences in the latency of mice at different time points(P<0.05);(2)The latency of the three groups was different(P<0.05).Compared with sham group,the latency of MIR-Control group was longer(P<0.05),and the latency of MIR-Sevopre group was shorter compared with MIR-Control group(P<0.05);(3)There
作者
王春晖
岳毅
刘少凯
刘小霞
柳元桢
张彦清
Chun-huiWang;Yi Yue;Shao-kai Liu;Xiao-xia Liu;Yuan-zhen Liu;Yan-qing Zhang(College of Anesthesiology,Shanxi Medical University,Taiyan,Shanxi 030001,China;Department of Neurosurgery,Shanxi General Hospotal of Chinese People's Armed Police Force,Taiyuan,Shanxi 030006,China;Department of Anesthesiology,The First Affiliated Hospital of Shanxi Medical University,Taiyuan,Shanxi 030001,China)
出处
《中国现代医学杂志》
CAS
北大核心
2022年第17期33-37,共5页
China Journal of Modern Medicine
基金
国家自然科学基金(No:81700327)
山西省基础研究计划项目(No:202103021224401)。
关键词
心肌缺血再灌注
小鼠
七氟醚
脂联素
认知功能
myocardial ischemia reperfusion
mice
sevoflurane
adiponectin
postoperative cognitive dysfunction
