摘要
为研究干旱胁迫对高海拔繁育藜麦幼苗抗旱性的影响,以高海拔扩繁前后藜麦为材料,分别用轻度干旱(持水量65%)、中度干旱(持水量45%)和重度干旱(持水量25%)处理藜麦幼苗,分别测定干旱胁迫处理后24、48、96 h及复水后幼苗的株高、氮平衡指数、叶绿素指数、类黄酮指数,探究干旱对藜麦幼苗生长及生理的影响。结果表明:1)干旱胁迫96 h后,在重度干旱下LL1和NL1的株高明显受到抑制,较CK分别减少了29.91%、22.83%,LL1地上部和地下部的各处理组干鲜质量均高于对照组CK;复水后LL1的W1组和W2组根的干鲜质量以及株高均高于NL1。2)干旱处理48 h,LL1叶绿素指数在中度干旱呈显著下降趋势,96 h后指标恢复;复水后各处理组下LL1藜麦幼苗叶片叶绿素指数变化趋势不显著。与对照相比,NL1中度干旱处理组叶绿素指数在干旱48 h呈显著下降趋势,干旱96 h仍呈下降趋势。复水后LL1叶绿素指数高于NL1。3)在不同干旱处理24 h和48 h,LL1类黄酮指数均高于对照组,复水后不同处理组LL1类黄酮指数高于NL1。4)在不同时间处理下LL1与NL1氮平衡指数随干旱程度增加呈持续上升趋势;复水后不同处理组LL1氮平衡指数皆高于NL1。5)LL1花青素指数随干旱程度增加先升后降,在中度干旱处理24 h下LL1与对照组相比增加21.43%;在干旱处理48 h时,随干旱程度增加,NL1花青素指数呈先升后降趋势。综上所述,高海拔扩繁后的陇藜1号(LL1)抗干旱能力明显优于高海拔扩繁前(NL1)。
This study was aimed to assess the effects of drought stress and subsequent rehydration on drought tolerance of the seedlings of quinoa propagated at high altitude.Two accessions of the quinoa variety Longli No.1 were used as experimental materials:one had been propagated at high altitude and the other had not,designated as LL1 and NL1,respectively.Quinoa seedlings of both NL1 and LL1 were treated with mild drought(W1,65%water holding capacity),moderate drought(W2,45%water holding capacity),and severe drought(W3,25%water holding capacity),while seedlings treated with normal irrigation were used as the control(CK).The effects of drought stress on quinoa seedling growth were evaluated by measuring the plant height,nitrogen balance index,chlorophyll content,and flavonoid content of seedlings at 24,48,and 96 h under drought stress treatment and after rehydration that followed 96 h of drought treatment.The results are as follows.Firstly,at 96 h of drought treatment,plant heights in W3 of both LL1 and NL1 significantly dropped by 29.91%and 22.83%,respectively,compared with CK;all drought treatments of LL1 exceeded CK in dry and fresh weights of above-and below-ground parts.After rehydration,the W1 and W2 of LL1 showed significantly higher plant height and dry and fresh weights of roots than those of NL1.Secondly,at 48 h of drought treatment,the W2 of LL1 had significantly lower chlorophyll content than CK,but the difference disappeared at 96 h.After rehydration,all treatments of LL1 had similar chlorophyll contents.The chlorophyll content in the W2 of NL1 showed a significant downward trend at 48 h and 96 h compared with CK,and after rehydration,LL1 had higher chlorophyll contents than NL1.Thirdly,at 24 h and 48 h,all drought treatments of LL1 had higher flavonoids contents than CK;they also had higher flavonoids contents than those of NL1 after rehydration.Fourthly,the nitrogen balance index of both LL1 and NL1 groups showed a continuous upward trend with intensifying drought stress at each time point of drought treat
作者
马建蓉
朱玉雪
朱永娟
梅艳桃
马国花
郭晓农
MA Jian-rong;ZHU Yu-xue;ZHU Yong-juan;MEI Yan-tao;MA Guo-hua;GUO Xiao-nong(College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030,China;Biomedical Research Center,Northwest Minzu University,Lanzhou 730030,China)
出处
《大麦与谷类科学》
2022年第4期14-20,共7页
Barley and Cereal Sciences
基金
西北民族大学2021年本科生创新项目(XBMU21118)。
关键词
藜麦
干旱胁迫
复水
生理特性
Chenopodium quinoa
Drought stress
Rehydration
Physiological characteristics
