摘要
通过分析岷江柏的迁地保护居群和野生居群的遗传多样性、遗传结构及居群间基因流,判断迁地保护岷江柏居群的遗传多样性水平,为其迁地保护提供理论基础。本研究利用GBS(Genotyping-by-Sequencing)测序技术获得的SNP位点对四川大渡河双江口岷江柏迁地保护移栽苗、苗圃播种苗及3个野生居群进行主成分分析(PCA分析)、聚类分析、分子进化树、遗传多样性和遗传结构分析。经过GBS测序共获得高质量Clean Data 118321514728 bp,并开发了1947047个tags,从中鉴定到了1259610个SNP位点。系统发育进化树显示大部分移栽岷江柏居群和野生岷江柏聚在一起,居群结构分析结果显示交叉验证错误率的谷值确定最优分群数为1。4个岷江柏居群的观测杂合度(H_(o))、期望杂合度(H_(e))、Shannon信息指数I(S_(hi))、近交系数(F_(is))、多态信息含量(P_(ic))的值分别为0.1815~0.2720、0.2232~0.3003、0.3310~0.4649、0.1780~0.2465和0.2722~0.3092,说明岷江柏居群的遗传多样性水平较高。移栽岷江柏居群的H_(e)=0.3003,S_(hi)=0.4649,岷江柏居群迁地保护居群遗传多样性总体水平略高于野生居群。野生岷江柏居群中白湾隧道(BW)_vs_松岗镇(SA)的遗传分化指数(F_(st))较大,基因流(N_(m))较小(F_(st)=0.091,N_(m)=2.496),而迁地保护的岷江柏居群与野生岷江柏居群没有明显的遗传分化,居群间的基因交流频繁(F_(st)<0.05,基因流N_(m)>4),说明没有明显的分群现象,岷江柏居群迁地保护居群遗传多样性较高。因此,移栽濒危植物是迁地保护过程中较好的方法,本文为以后野生岷江柏迁地保护提供参考,为其他树木种质资源的保存提供理论依据。
To evaluate the genetic diversity level and provide theoretical basis of ex-situ conservation populations of Cupressus chengiana,the genetic diversity,genetic structure,and gene flow of ex-situ conservation populations and wild populations of C.chengiana were studied respectively.The principal component analysis(PCA),cluster analysis,construction of molecular evolutionary tree,genetic diversity and genetic structure of three wild populations and ex-situ conservation populations based on SNP locus were obtained respectively from Genotyping-by-Sequencing technology(GBS)sequencing.The results showed that high quality clean data 118321514728 bp was obtained by GBS sequencing,and 1947047 tags were developed.After being screened,a total of 1259610 of high-quality SNP sites were retained.The valley value of cross validation error rate determined that the optimal number of clusters was 1.The phylogenetic tree indicated that most of individuals ex-situ conservation populations and wild populations were clustered together.Both of combined ex-situ conservation populations and wild populations of C.chengiana had higher genetic diversity,and their value of heterozygosity(H_(o)),expected heterozygosity(H_(e)),Shannon information index(S_(hi)),inbreeding coefficient(F_(is)),and percentage of polymorphic loci(P_(ic))were 0.1815-0.2720,0.2232-0.3003,0.3310-0.4649,0.1780-0.2465 and 0.2722-0.3092,respectively.Genetic diversity of ex-situ conservation cultivated populations was relatively higher than the wild populations of C.chengiana,the value of H_(e) and S_(hi) was 0.3003 and 0.4649,respectively.The wild populationsof C.chengiana of Baiwan tunnel(BW)_vs_Songgang town(SA)had the greater genetic differentiation and the gene flow(N_(m))were lower than other populations(F_(st)=0.091,N_(m)=2.496).The results indicated that genetic differentiation had not occurred among these C.chengiana populations,and the gene had exchanges frequently among the ex-situ conservation populations and wild populations of C.chengiana(F_(st)<0.05,N_(m)>4)
作者
常二梅
刘建锋
黄跃宁
李红丽
单冰燕
江泽平
赵秀莲
CHANG Ermei;LIU Jianfeng;HUANG Yuening;LI Hongli;SHAN Bingyan;JIANG Zeping;ZHAO Xiulian(Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091;State Forestry Administration Key Laboratory of Tree Breeding and Cultivation,Beijing 100091;Beijing Forestry University,Beijing 100083;Agriculture and Rural Bureau of Yongnian District,Handan,Hebei 056011;Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091)
出处
《植物研究》
CAS
CSCD
北大核心
2022年第5期772-779,共8页
Bulletin of Botanical Research
基金
中国林业科学研究院林业研究所横向课题(C258)。
关键词
岷江柏
野生居群
迁地保护居群
GBS
遗传多样性
遗传结构
Cupressus chengiana
wild population
ex-situ conservation population
GBS
genetic diversity
genetic structure
