摘要
为了研究青海云杉针叶内生真菌群落结构多样性,寻找可能导致青海云杉针叶枯黄的原因,本研究采用高通量测序技术对两组健康及发病后的青海云杉针叶进行测序,并分析其内生真菌多样性和群落结构变化。结果显示4个样本共得到665个可以用于物种分类的运算分类单位(operational taxonomic units,OTU),其中共有的OTU数为149个。健康组和发病组间真菌群落结构差异较大,同一组内不同样本之间的相似性较高;两组样本优势菌群也存在不同,在属分类水平上,健康组优势菌群为球腔菌属(Mycosphaerella)、枝孢属(Cladosporium)和葡萄孢属(Botrytis),发病组优势菌群为枝孢属(Cladosporium)、Sarcinomyces和Rachicladosporium;物种相关性分析发现球腔菌属(Mycosphaerella)和枝孢属(Cladosporium)间存在负相关;发病组样本中球腔菌属(Mycosphaerella)菌群占比大幅度降低,枝孢属(Cladosporium)菌群在针叶内优势生长成为了丰度最高的菌群,可能与云杉针叶枯黄相关。以上研究结果为青海云杉内生真菌群落结构多样性及叶枯病原菌鉴定提供了参考依据。
To study the structural diversity of the endophytic fungal community of Picea crassifolia Kom.needles and to search for possible causes of needle yellowing in Picea crassifolia Kom.,this study was conducted to analyze the diversity and community structure of endophytic fungi of Picea crassifolia Kom.needles in healthy and susceptible groups using high-throughput sequencing technology.The results showed that a total of 665 OTUs could be used for species classification from 4 samples,among which 149 OTUs were in common.There were significant differences in fungal community structure between healthy group and disease group,and there were high similarities among different samples in the same group.There were also differences in the dominant groups of the two groups of samples,with the dominant groups in the healthy group being Mycosphaerella,Cladosporium and Botrytis;in the morbid group,Cladosporium,Sarcinomyces and Rachicladosporium at the genus classification level.Species correlation analysis revealed a negative correlation between the genera Mycosphaerella and Cladosporium.The percentage of Mycosphaerella in the samples of the disease group was significantly reduced,and Cladosporium was the most abundant bacterium in the needles,which may cause the wilt disease in spruce needles.These results provides a reference for the structural diversity of endophytic fungal communities and the identification of leaf blight pathogens in Picea crassifolia Kom.
作者
方泰军
白露超
Fang Taijun;Bai Luchao(Collge of Agriculture and Animal Husbandry,Qinghai University,Xining,810016)
出处
《分子植物育种》
CAS
北大核心
2022年第15期5198-5204,共7页
Molecular Plant Breeding
基金
青海省“昆仑英才·高端创新创业人才”项目
青海省云杉属树种优良种质资源分子鉴定及良种选育项目(青财预字[2020]63号)共同资助。
关键词
青海云杉
高通量测序
内生真菌
多样性
Picea crassifolia Kom.
High throughput sequencing
Endophytic fungi
Diversity
