摘要
为探明菌塘土壤营养变化与其微生物多样性改变的相关性,以印度块菌成熟期10月到次年2月的菌塘原生土壤为研究对象,通过连续性土壤营养指标和微生物多样性检测,分析印度块菌成熟期不同阶段菌塘土壤营养和微生物多样性变化规律及三者关系。结果显示:成熟期菌塘土壤的真菌、细菌多样性先降后升,Shannon指数的最大值出现在次年2月,并与有效磷有较高相关性;真菌的物种数目变异系数比细菌高,优势菌门为子囊菌门,优势菌属为块菌属和被孢霉属,两者是竞争关系,其中块菌属与全磷、速效钾、pH呈正相关;细菌的优势菌门是变形菌门,基因丰度较高的酸杆菌RB41与土壤营养指标均呈正相关,慢生根瘤菌基因丰度与有机质、全氮、碱解氮含量呈正相关。可得结论:在块菌仿生栽培中,理论上可通过补充土壤磷、钾含量及调节pH为弱碱性,在土壤菌群中提升酸杆菌RB41和慢生根瘤菌的优势等途径来实现成熟期块菌生长增效作用的可能性,但其增效程度有待进一步验证。
In this study,the original soil of the fungus pond in the mature period of Tuber indicum from October to February of the following year was used as the research object.Through continuous soil nutrient index and microbial diversity detection,the change rules of soil nutrition and microbial diversity in the pond of Tuber indicum at different stages of maturity,and the relationship were analyzed.The results showed that the fungal and bacterial diversity of the pond soil at the mature stage first decreased and then increased.The maximum value of Shannon index appeared in February of the following year,and it was highly correlated with available phosphorus;The coefficient of variation of the species number of fungi was higher than that of bacteria.The dominant fungal phylum was Ascomycota,and the dominant fungal genera were Truffle and Mortierella.Among them,truffle is positively correlated with total phosphorus,available potassium,and pH;The dominant bacterial phylum was Proteobacteria.Acidobacter RB41 with higher gene abundance was positively correlated with soil nutrient indexes,and the gene abundance of Bradyrhizobia was positively correlated with the contents of organic matter,total nitrogen and alkaline hydrolyzed nitrogen.Therefore,in the bionic cultivation of truffles,the synergistic effect can be achieved by supplementing the phosphorus and potassium content of the soil,adjusting the pH to weak alkaline,and enhancing the advantages of Acidobacter RB41 and Brachyrhizobia in the soil flora,but its efficiency needs to be further verified.
作者
曾维军
王晶
杨彝华
和耀威
刘琼波
康超
杨玲
ZENG Wei-jun;WANG Jing;YANG Yi-hua;HE Yao-wei;LIU Qiong-bo;KANG Chao;YANG Ling(Guizhou Institute of Biology,Guiyang Guizhou 550025,P.R.China;Chuxiong Institute of Forestry and Grassland Science,Chuxiong Yunnan 675005,P.R.China)
出处
《西部林业科学》
CAS
北大核心
2022年第4期26-33,共8页
Journal of West China Forestry Science
基金
黔科合服企2018-4002“贵州主要野生食用菌产业化技术集成及示范推广”
贵州省高层次创新型人才培养“千人创新创业人才”
黔科合基础—ZK[2022]一般283“基于代谢组学榛子与块菌共生机理的研究”
黔科合支撑2019-2336“贵州林下特色经济野生食用菌保育及仿生态栽培技术研究”。
