摘要
利用RT-PCR技术从延谷11号谷子中克隆SiPRR73基因,通过系统分析SiPRR73组织表达特异性,对不同光周期处理、不同光温组合处理以及5种非生物胁迫处理的响应特点,揭示光周期、温度对SiPRR73的调控模式及SiPRR73对非生物胁迫的应答模式。结果表明,从延谷11号克隆得到SiPRR73基因2928 bp的cDNA序列,包含2283 bp的CDS区,编码760个氨基酸;SiPRR73蛋白与C4家族作物糜子、哈氏黍、高粱、玉米PRR73蛋白具有较近的进化关系。SiPRR73在顶端第2片叶中表达水平最高,在根、茎、穗部表达水平较低。短日照、长日照条件下该基因均表现光照期表达水平高于黑暗期的特点,且整个营养生长期短日照条件下表达水平明显低于长日照,SiPRR73受光诱导表达和光周期调控。温度决定SiPRR73表达峰的数目,光周期决定SiPRR73表达峰出现的时间,光周期和温度共同参与了SiPRR73的表达调控。PEG和低温胁迫总体上诱导SiPRR73表达,NaCl在胁迫早期诱导该基因表达,后期总体表现为抑制状态,Fe胁迫则早期抑制该基因表达,后期总体诱导其表达,SiPRR73对ABA胁迫的响应特点接近NaCl。以上研究表明,谷子SiPRR73表达具有光依赖性,光周期和温度以互作模式调控SiPRR73表达,推测SiPRR73参与了谷子对不同光温环境的适应性调节过程,并且在应对干旱、低温、ABA、NaCl、Fe胁迫过程中发挥了一定作用。
The SiPRR73 gene was cloned from Yangu 11 using RT-PCR technology,and through analyzing tissue-specific expression,responsive features of SiPRR73 to different photoperiods,photo-thermal combinational treatments and five abiotic stress treatments,the regulation mode of photoperiod and temperature on SiPRR73,and the responsive pattern of SiPRR73 to abiotic stresses in foxtail millet were explored.The results showed that totally 2928 bp cDNA sequence of SiPRR73 was obtained from Yangu 11,which included 2283 bp CDS region,encoding 760 amino acids.The SiPRR73 proteins of C4 crops including Panicum miliaceum,Panicum hallii,Sorghum bicolor and Zea mays showed relatively close relationship with SiPRR73.The second parietal leaf was the highest expression tissue of SiPRR73,but the expression level at root,stem and panicle tissues was relatively lower.The expression level of SiPRR73 was higher at light period than that at dark period under both short-day and long-day conditions,and during the whole vegetative growth phase,SiPRR73 showed higher expression level under long-day compared to short-day,which indicated that the expression of SiPRR73 was induced by light and controlled by photoperiod.The temperature determined expression peak number of SiPRR73 and the photoperiod determined occurrence time of expression peaks,so temperature and photoperiod participated in regulating of SiPRR73 expression mutually.PEG and low temperature stresses induced SiPRR73 expression totally,NaCl induced SiPRR73 expression at early stress stage,but inhibited it at later stress stage.Fe stress inhibited SiPRR73 expression at early stage,but induced it at later stage.ABA stress caused the close responsive feature of SiPRR73 to NaCl.This study indicated that SiPRR73 showed light-dependent expression feature,and photoperiod and temperature regulated SiPRR73 by interaction pattern,suggesting that SiPRR73 participated in adaptability regulation process to different photo-thermal conditions and might play a certain role in coping with drought,low tem
作者
闫留延
李剑峰
张世文
张博
王永芳
张小梅
祖超凡
王振山
桑璐曼
何占祥
贾小平
董志平
YAN Liuyan;LI Jianfeng;ZHANG Shiwen;ZHANG Bo;WANG Yongfang;ZHANG Xiaomei;ZU Chaofan;WANG Zhenshan;SANG Luman;HE Zhanxiang;JIA Xiaoping;DONG Zhiping(College of Agriculture,Henan University of Science and Technology,Luoyang 471023,China;Institute of Millet Crops,Hebei Academy of Agriculture and Forestry Sciences,National Millet Improvement Center,Shijiazhuang 050035,China)
出处
《华北农学报》
CSCD
北大核心
2022年第4期11-19,共9页
Acta Agriculturae Boreali-Sinica
基金
国家自然科学基金项目(31471569)。
关键词
谷子
PRR73
光周期
温度
非生物胁迫
Foxtail millet
PRR73
Photoperiod
Temperature
Abiotic stress
