摘要
反硝化过程是导致土壤N_(2)O排放的主要过程之一。为明确苯甲酸对反硝化过程中N_(2)O排放的影响,本研究通过外援添加苯甲酸进行室内反硝化细菌铜绿假单胞菌(Pseudomonas aeruginosa)代谢活性及土壤培养试验,探讨了苯甲酸对反硝化细菌代谢活性及反硝化过程N_(2)O排放的影响。结果表明:(1)土壤N_(2)O排放特征受苯甲酸添加量影响显著,其中在低浓度(1~2 mmol/kg)时呈现“双峰”、高浓度时(4~6 mmol/kg)呈“单峰”的特征;(2)苯甲酸对土壤N_(2)O累积排放量的影响呈现出低浓度促进(增幅达136.4%)、高浓度抑制(降幅为43.0%~64.1%)的特征;(3)苯甲酸对土壤NO_(3)-转化表现为前期抑制后期促进的特征,这与其铜绿假单胞菌代谢活性的影响完全吻合。综上,合理应用苯甲酸可降低土壤N_(2)O排放,但其适宜用量与N_(2)O减排机理仍需进一步研究。
Denitrification is one of the main processes of soil N_(2)O emission from soil.In this study,bacterial metabolic activity of Pseudomonas aeruginosa and soil culture experiments were carried out to clarify the effect of benzoic acid on N_(2)O emission from denitrification process.The results show that:(1)The characteristics of soil N_(2)O emission were strongly affected by the concentration of benzoic acid,which showed“double peak”at low concentration(1-2 mmol/kg)and“single peak”at high concentration(4-6 mmol/kg).(2)Application of benzoic acid promotes N_(2)O emission at low concentration(an increase of 136%)and inhibits N_(2)O emission at high concentration(43.0%–64.1%).(3)Application of benzoic acid inhibited the transformation of soil NO_(3)-in the early stage and promoted it in the later stage,which was completely consistent with its effect on the metabolic activity of Pseudomonas aeruginosa.In conclusion,the rational application of benzoic acid can reduce soil N_(2)O emission,but its appropriate dosage and N_(2)O emission reduction mechanism still need to be further studied.
作者
贾文慧
张新星
李睿琦
高志岭
JIA Wenhui;ZHANG Xinxing;LI Ruiqi;GAO Zhiling(College of Resource and Environmental Sciences,Hebei Agricultural University/Key Laboratory for Farmland Eco-Environment of Hebei Province,Baoding 071000,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2022年第4期79-85,共7页
Journal of Hebei Agricultural University
基金
河北农业大学科研发展基金(3003181).
关键词
苯甲酸
氧化亚氮
排放
土壤反硝化
室内培养
benzoic acid
nitrous oxide
emission
soil denitrification
incubation
