摘要
目的了解中国乙型肝炎病毒(hepatitis B virus,HBV)基因组序列的免疫逃逸突变、耐药突变和基因组进化信息。方法选取1998-2021年在GenBank中的中国HBV全基因组序列信息作为分析对象,采用MAFFT软件进行聚类分析,使用在线工具Gen2pheno进行免疫逃逸突变和耐药突变分析,使用BEAST 1.10.4做序列的时间进化分析。结果5426条序列纳入分析集,分布于我国19个省份,C型占比最高(59.1%,3211/5426)。其次为B型(33.7%,1833/5426)。有764条(14.1%,764/5426)序列发生了免疫逃逸突变,98.1%的序列至少发生了1处反转录酶编码区域突变。中国大部分系列的进化根在公元1801年左右。结论中国HBV耐药突变率较高,HBV基因组进化缓慢。
Objective To understand immune escape mutation,drug resistance mutation,and genome evolution information of HBV genome sequence in China.Methods The whole genome sequence information of HBV in China submitted in GenBank from 1998 to 2021 was selected as the object for analysis.MAFFT method was used for cluster analysis.Analysis of immune escape and drug-resistant mutations was performed using the online tool Gen2pheno.The BEAST 1.10.4 was used for analysis the time evolution of HBV sequences.Results A total of 5426 sequences were included in the dataset and distributed in 19 provinces of China.Type C accounted for the highest proportion(59.1%,3211/5426),followed by type B(33.7%,1833/5426).Immune escape mutations were found in 764 sequences(14.1%,764/5426).At least one reverse transcriptase region mutation occurred in 98.1%of the sequences.The evolutionary roots of most HBV sequences in China date from around 1801 AD.Conclusion HBV-resistant mutation rate is high in China.HBV genomes evolve slowly.
作者
郭永豪
豆巧华
刘倩
杨建辉
吕宛玉
丰达星
僧明华
张延炀
赵东阳
Guo Yonghao;Dou Qiaohua;Liu Qian;Yang Jianhui;Lyu Wanyu;Feng Daxing;Seng Minghua;Zhang Yanyang;Zhao Dongyang(Medicine Key Laboratory of Immunoprophylaxis,Henan Center for Disease Control and Prevention,Zhengzhou 450016,China)
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2022年第8期1309-1314,共6页
Chinese Journal of Epidemiology
基金
河南省自然科学基金(222300420466)
河南省医学科技攻关项目(2018010028)。
关键词
乙型肝炎
基因组耐药和免疫逃逸突变
基因组进化
Hepatitis B
Genomic drug resistance and immune escape mutations
Genomic evolution
