摘要
目的:探讨持续运动和间歇负重运动对2型糖尿病(T2DM)骨骼肌组织细胞形态、骨骼肌Rab5 mRNA及蛋白表达、骨骼肌糖代谢的影响。方法:SD大鼠选取8只为空白对照组(CR),其他采用高脂高糖饲料喂养6周后,腹腔注射STZ(35 mg/kg)构建T2DM模型。选取24只T2DM分3组(n=8),分别为:T2DM模型组(DRM)、持续运动组(DCRE)、间歇负重运动组(DWRE)。持续运动方案:为前1~2周准备活动15 m/min(10 min)、运动20 m/min(40 min)、整理活动15 m/min(10 min),后3~8周为18 m/min(10 min)、25 m/min(40 min)、15 m/min(10 min);间歇负重运动方案:采用负荷重量为15%(1~2周)、30%(3~4周)、45%(5~8周),运动均为15 m/min(5 min),共12组,组间休息3 min。8周后,通过HE观察骨骼肌病理形态变化,qRT-PCR检测骨骼肌Rab5、葡萄糖转运酶4(GLUT4)的mRNA表达,免疫荧光组化技术及Western blot检测骨骼肌Rab5的蛋白表达,ELISA检测血浆Rab5和糖化血红蛋白(GHb)浓度。结果:相比CR,DRM存在骨骼肌病理损伤,骨骼肌Rab5mRNA及蛋白表达、GLUT4 mRNA表达均降低(P<0.01),血浆Rab5和GHb均显著升高(P<0.01);与DRM比较,DCRE、DWRE骨骼肌病理损伤均显著减轻,骨骼肌Rab5 mRNA及蛋白表达、GLUT4 mRNA表达均升高(P<0.05,P<0.01),血浆Rab5和GHb降低(P<0.01);DCRE与DWRE组间均无统计学差异(P>0.05)。结论:2种运动方式均能改善2型糖尿病大鼠骨骼肌病理损伤,并可通过提高骨骼肌Rab5基因和蛋白表达从而增强GLUT4转运能力,缓解骨骼肌糖代谢稳态失衡,但2种运动方式对骨骼肌Rab5蛋白和糖代谢的影响无明显差异性。
Objective:To investigate the effects of continuing exercise and load-bearing interval exercise on skeletal muscle tissue cell morphology,Ras-related proteins 5(Rab5)mRNA and protein expression and glucose metabolism in skeletal muscle of type 2 diabetic mellitus(T2DM)rats.Methods:Eight SD rats were selected as controls group(CR),the others SD rats were fed with high fat and high sugar diet for 6 weeks before injecting STZ(35 mg/kg)to construct the T2DM model.Twenty-four T2DM rats were randomly devided into T2DM model group(DRM),continuing exercise group(DCRE)and load-bearing interval exercise group(DWRE),8 rats in each group.DCRE exercise protocol,that was 15 m/min(10 min),20 m/min(40 min),15 m/min(10 min),during the first 1~2 weeks,and 18 m/min(10 min),25 m/min(40 min),15 m/min(10 min),during the second 3~8 weeks.DWRE exercise protocol:load weight 15%/1~2 weeks,30%/3~4 weeks,45%/5~8 weeks,with 15 m/min(5 min),12 groups and 3 min rest between groups.After 8 weeks,pathological and morphological changes of skeletal muscle were observed by HE.Rab5 and Glucose transporte 4(GLUT4)mRNA expressions of skeletal muscle were tested by qRT-PCR.Rab5 protein expression in skeletal muscle was tested by immunofluorescence histochemistry and Western blot,and plasma Rab5 and Glycosylated Hemoglobin(GHb)concentrations were detected by ELISA.Results:Comparison with CR,DRM showed pathological damage of skeletal muscle,the expressions of Rab5 mRNA,protein and GLUT4 mRNA were all decreased in skeletal muscle(P<0.01),the serum levels of Rab5 and GHb were both significantly elevated(P<0.01).Comparison with DRM,both DCRE and DWRE significantly improved pathological damages of skeletal muscle,the expressions of Rab5 mRNA,protein and GLUT4 mRNA were all increased in skeletal muscle(P<0.05,P<0.01),the serum levels of Rab5 and GHb were decreased(P<0.05,P<0.01),and there was no statistical difference between DCRE and DWRE groups(P>0.05).Conclusion:Two exercise modes can improve the pathological injury of skeletal muscle in type 2 diabetic rat
作者
关东如
方明
朱嫚子
王克
崔勇
柏友萍
GUAN Dong-ru;FANG Ming;ZHU Man-zi;WANG Ke;CUI Yong;BAI You-ping(Physical College of Anhui Normal University,Wuhu 241002;Qingdao Sports Industry Development Center,Qingdao 266071;Wannan Medical College,Wuhu 241003,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2022年第3期207-211,232,共6页
Chinese Journal of Applied Physiology
基金
安徽省高校自然科学基金重点(KJ2017A238)。
关键词
T2DM
骨骼肌
糖代谢
Rab5
持续运动
间歇负重运动
type 2 diabetic mellitus
skeletal muscle
glucose metabolism
Rab5
continuing exercise
load-bearing interval exercise
