摘要
目的:研究中药饮片金银花、菊花微生物限度检查方法。方法:按照《中国药典》2020年版规定,测定试验菌株的回收率,研究需氧菌总数、霉菌和酵母菌总数的计数方法和控制菌检查方法,并对方法加以试验验证。结果:试验结果表明,用常规法开展实验,需氧菌总数、霉菌和酵母菌总数方法验证试验,供试品对照组的试验菌回收率在0.5~2.0范围内;控制菌检查,实验组检出阳性试验菌,阴性对照没有菌落生长。结论:该方法简便、准确、安全、可靠,可用于中药饮片金银花、菊花的微生物限度检查。
Objective:To study the microbial limit test of honeysuckle and chrysanthemum.Methods:According to the“Chinese Pharmacopoeia”(2020 edition),determine the recovery rate of the tested strains,study the counting method of the total number of aerobic bacteria,the total number of mould and yeast,and the checking method of the control bacteria.Results:The routine method was used to carry out the experiment,results showed that the total number of aerobic bacteria,mold and yeast method validation test,test bacteria recovery in the control group in the range of 0.5-2.0.Control bacteria examination,the experimental group detected positive test bacteria,negative control no colony growth.Conclusion:The method is simple,accurate,safe and reliable,and can be used for microbial limit test of honeysuckle and chrysanthemum.
作者
尹良军
杨晓莉
王文娟
王宏杰
YIN Liangjun;YANG Xiaoli;WANG Wenjuan;WANG Hongjie(Tongchuan food and drug inspection and testing center,Tongchuan Shaanxi 727031,China;Shaanxi Institute for Food and Drug,Xi'an Shaanxi 710065,China;NMPA Key Laboratory of Pharmaceutical Microbiology Detection Technology for Drugs,Xi'an Shaanxi 710065,China)
出处
《药品评价》
CAS
2022年第10期585-588,共4页
Drug Evaluation
基金
陕西省药品科学监管和监管科学研究项目(SXYJ202108)。
关键词
中药饮片
金银花
菊花
微生物限度检查
常规法
Chinese herbal pieces
Honeysuckle
Chrysanthemum
Microbial limit test
Routine method
