摘要
溃疡性结肠炎(UC)发病率在全球范围内不断上升,已成为严重的健康问题。诺丽果浆作为新食品原料含有丰富的活性物质,具有多种对人体有益的保健功能,如抗炎、抑菌和降血脂等。将小鼠分为3组,分别为空白组(C)、模型组(M)和诺丽发酵汁(FNJ)饲养组(N)。M组和N组给予葡聚糖硫酸钠(DSS)灌胃以诱导结肠炎,1周后对N组小鼠进行FNJ灌胃,两周后处死并测量相关指标。结果表明:N组小鼠疾病活动指数明显降低,结肠组织病理学改变明显减少。同时,FNJ处理的小鼠血清促炎细胞因子降至对照组水平。与M组相比,FNJ能调控结肠炎小鼠肠道菌群组成,降低厚壁菌门的相对丰度,并增加拟杆菌门的相对丰度,提示FNJ能介导肠道菌群缓解小鼠DSS结肠炎,研究结果可为开发FNJ的营养功能和药用价值提供科学依据,为维持肠道稳态和缓解结肠炎提供新途径。
Ulcerative colitis(UC)incidence is rising globally,and has become a serious health problem in Asia.Noni(Morinda citrifolia L.),a kind of medicinal and edible plant,is rich in active substances.Studies have shown that noni fruit has a variety of beneficial health functions such as anti-inflammatory,antibacterial and lowering blood lipids.In this study,mice were divided into three groups:control group(C),model group(M),and naturally fermented noni juice(FNJ)treated group(N).Groups M and N were gavaged with dextran sulfate sodium(DSS)to induce colitis.One week later,mice in group N were gavaged with FNJ.After two weeks,they were killed and related indicators were measured.The results showed that the disease activity index of mice in group N was significantly reduced,and the histopathological changes of colon were alleviated.Meanwhile,serum pro-inflammatory cytokines in FNJ-treated mice were also modulated to normal levels.Compared with the M group,FNJ can also regulate the composition of the intestinal microbiota of mice with colitis,with the relative abundance of Firmicutes reducing and that of Bacteroides increasing.This research provides a scientific basis for developing the nutritional function and medicinal value of FNJ,and supplies new ideas for the intestinal homeostasis and treatment of colitis.
作者
曲泰齐
张家超
汪瑞敏
刘四新
任发政
李从发
Qu Taiqi;Zhang Jiachao;Wang Ruimin;Liu Sixin;Ren Fazheng;Li Congfa(College of Food Science and Engineering,Hainan University,Haikou 570228;Department of Nutrition and Health,China Agricultural University,Beijing 100193)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2022年第8期118-126,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金地区项目(31760456)。
关键词
诺丽发酵汁
肠道菌群
结肠炎
高通量测序
fermented noni juice
intestinal microbiota
colitis
high-throughput sequencing
