摘要
目的 探讨血小板内皮聚集受体-1(PEAR1)基因多态性与急性肺栓塞(APE)合并肺部感染的关联。方法选择福建医科大学附属闽东医院2018年12月-2020年12月收治的APE患者102例,根据住院期间是否发生肺部感染,分为肺部感染组39例与未感染组63例;检测其凝血功能指标凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(FIB);采用实时荧光定量聚合酶链式反应检测PEAR1基因rs12041331、rs2768759位点单核苷酸多态性。结果 肺部感染组与未感染组rs2768759位点基因型分布比较,无统计学差异,肺部感染组rs12041331位点AA型频率高于未感染组(P<0.05);肺部感染组凝血功能指标PT、APTT和FIB水平均高于未感染组,TT水平低于未感染组(P<0.05);肺部感染组PEAR1 rs12041331位点携带AA型患者PT、APTT和FIB水平高于GG/GA型,TT水平低于GG/GA型(P<0.05)。结论 本地区APE患者住院期间肺部感染易感性增加可能与PEAR1 rs12041331位点携带AA型有关,该位点可能通过影响凝血功能而导致肺部抗感染能力的下降。
OBJECTIVE To explore the relationship between platelet endothelial aggregation receptor-1(PEAR1) polymorphism and pulmonary infection in patients with acute pulmonary embolism(APE). METHODS Total of 102 APE patients admitted to the Mindong Hospital of Fujian Medical University from Dec 2018 to Dec 2020 were enrolled. According to whether lung infection occurred during the hospitalization, they were divided into 39 cases in the lung infection group and 63 cases in the non-infected group. Coagulation function indicators including prothrombin time(PT), activated partial thromboplastin time(APTT), thrombin time(TT) and fibrinogen(FIB) were detected. Real-time fluorescent quantitative polymerase chain reaction was used to detect single nucleotide polymorphism in PEAR1 genes sites(rs12041331, rs2768759). RESULTS There was no significant difference in the genotype distribution of rs2768759 between the pulmonary infection group and the uninfected group. The frequency of AA at rs12041331 site in the lung infection group was significantly higher than that in the uninfected group(P<0.05). The coagulation function indexes such as PT, APTT and FIB in the lung infection group were significantly higher than those in the uninfected group, while TT level was significantly lower than that in the uninfected group(P<0.05). The PT, APTT and FIB levels in patients with type AA at PEAR1 rs12041331 in the lung infection group were significantly higher than those with the GG/GA type, while the level of TT was lower(P<0.05). CONCLUSION The increase of susceptibility to pulmonary infection in the APE patients may be associated with the AA genotype at rs12041331 locus of PEAR1, and the locus may lead to the decline of pulmonary anti-infection ability by affecting the coagulation function.
作者
詹祥辉
邓朝胜
宋彬
林辉
郭良华
郭国华
曾林淼
肖建宏
ZHAN Xiang-hui;DENG Zhao-sheng;SONG Bin;LIN Hui;GUO Liang-hua;GUO Guo-hua;ZENG lin-miao;XIAO Jian-hong(Mindong Hospital of Ningde City,Ningde,Fujian 355000,China;不详)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2022年第3期399-402,共4页
Chinese Journal of Nosocomiology
基金
福建省财政厅计划基金资助项目(2018DF030008)。
关键词
血小板内皮聚集受体-1
单核苷酸多态性
急性肺栓塞
肺部感染
关联性
Platelet endothelial aggregation receptor-1
Single nucleotide polymorphism
Acute pulmonary embolism
Pulmonary infection
Susceptibility
