摘要
光是植物获取能量的主要来源,杜衡为典型的阴生药用植物,一般生长于灌木林下沟边阴湿之地,该实验探究遮阴对杜衡生长、生理特性及能量代谢的影响,为杜衡的人工种植提供参考和指导。该实验在80%遮阴、60%遮阴、40%遮阴、20%遮阴和无遮阴处理下种植杜衡,于生长旺盛期测定其荧光参数、光合参数、光合色素含量、超微结构等光合生理特性,以及线粒体电子传递链(ETC)合酶和营养物质含量。结果表明:光合色素含量、叶绿素荧光参数与净光合速率(P_(n))均与遮阴成正相关;超微结构在20%~40%遮阴处理下受到损害,叶绿体膨胀解体,基质片层及基粒片层混乱,嗜锇颗粒和淀粉粒增多;线粒体复合体Ⅰ脱氢酶(NADH脱氢酶)、琥珀酸脱氢酶(SDH)、细胞色素C氧化酶(CCO)、ATP合酶活性均与遮阴正相关;随遮阴的下降,营养物质中总糖及蛋白质含量先上升后下降,60%遮阴处理下含量最高。综上,60%~80%遮阴处理下叶绿体和线粒体结构更完整,能量代谢旺盛,光能转换效率高,能够较好地吸收和利用光能,合成较多营养物质,更适合杜衡生长发育。
Light is the main source for plants to obtain energy.Asarum forbesii is a typical shade medicinal plant,which generally grows in the shady and wet place under the bushes or beside the ditches.It can grow and develop without too much light intensity.This experiment explores the effects of shading on the growth,physiological characteristics and energy metabolism of A.forbesii,which can provide reference and guidance for its artificial planting.In this experiment,A.forbesii was planted under 80%,60%,40%,20%and no shade.During the vigorous growth period,the photosynthetic physiological characteristics such as fluorescence parameters,photosynthetic parameters,photosynthetic pigment content and ultrastructure,as well as the content of mitochondrial electron transport chain(ETC)synthase and nutrients were measured.The results showed that the photosynthetic pigment content,chlorophyll fluorescence parameters and net photosynthesis rate(P_(n))decreased with the decrease of shading.Under 20%-40%shading treatment,the plants had damaged ultrastructure,expanded and disintegrated chloroplast,disordered stroma lamella and grana lamella,and increased osmiophi-lic granules and starch granules.The activities of nicotinamide adenine dinucleotide dehydrogenase(NADH),succinate dehydrogenase(SDH),cytochrome C oxidoreductase(CCO)and adenosine triphosphate(ATP)synthasewere positively related to light intensity.With the reduction of shading,the content of total sugar and protein in nutrients increased first and then decreased,and the content was the highest under 60%shade.In conclusion,under 60%-80%shading treatment,the chloroplast and mitochondria had more complete structure,faster energy metabolism,higher light energy-conversion efficiency,better absorption and utilization of light energy and more nutrient synthesis,which was more suitable for the growth and development of A.forbesii.
作者
陆李仙
史红专
郭巧生
赵坤
常淮阳
邹洁
郭丽媛
杨兆瑞
LU Li-xian;SHI Hong-zhuan;GUO Qiao-sheng;ZHAO Kun;CHANG Huai-yang;ZOU Jie;GUO Li-yuan;YANG Zhao-rui(Institute of Medicinal Materials,Nanjing Agriculural University,Nanjing 210095,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第15期4048-4054,共7页
China Journal of Chinese Materia Medica
基金
江苏现代农业产业技术体系建设项目(JATS[2021]460,JATS[2021]289)。
关键词
遮阴
杜衡
光合生理
能量代谢
shade
Asarum forbesii
photosynthetic physiology
energy metabolism
