摘要
为探究枯草芽孢杆菌添加剂对生物絮团菌群结构、絮团营养以及生物絮团培养过程中水质的影响,在生物絮团反应器中添加4种不同剂量的枯草芽孢杆菌。生物絮凝系统构建过程中,枯草芽孢杆菌的添加剂量分别为A组10~6 CFU/mL、B组10~5 CFU/mL、C组10~4 CFU/mL和D组0 CFU/mL。结果表明:生物絮凝系统构建过程中,枯草芽孢杆菌添加剂量对絮团总悬浮颗粒物(total suspended solids,TSS)、5 min内絮团沉降体积(floc volume,FV)影响显著;对絮团氨基酸含量、粗蛋白含量、絮团碳氮比影响显著,对絮团粗脂肪,粗灰分含量影响不显著;添加枯草芽孢杆菌絮团中氨基酸、粗蛋白含量高于不添加枯草芽孢杆菌组;枯草芽孢杆菌添加剂量对絮团门、属水平菌群差异物种相对丰度有显著影响,增加枯草芽孢杆菌添加剂浓度,絮团中芽孢杆菌浓度也随之升高。枯草芽孢杆菌能在生物絮团上得到有效富集,在生物絮凝系统构建过程中添加枯草芽孢杆菌能提高絮团粗蛋白和氨基酸含量,有利于为养殖对象提供蛋白源。
In order to explore the effect of adding Bacillus subtilis on the microbial community structure,nutrition of bioflocs and water quality in the process of system construction,the biofloc technology-aquaculture system was completed,the additive concentration of B.subtilis was group A,10~6 CFU/mL,group B,10~5 CFU/mL,group C,10~4 CFU/mL,group D,0 CFU/mL.The experimental results showed that:The concentration of B.subtilis had significant effect on the concentration of TSS and FV-30;The concentration of B.subtilis had a significant effect on the amino acid content and C/N of the floc;The concentration of B.subtilis had no significant effect on crude lipid and ash content;The amino acid and crude protein contents of adding B.subtilis groups were higher than those of the non-added group;The additive of B.subtilis had a significant effect on the relative abundance at phylum and genus level of bacteria;and increasing the concentration of B.subtilis additive led to an increase in the concentration of Bacillus in the biofloc.B.subtilis can be effectively enriched on bioflocs.The addition of B.subtilis can effectively improve the nutrient content of bioflocs,which is conducive to providing protein source for aquaculture.
作者
贺希
罗国芝
谭洪新
HE Xi;LUO Guozhi;TAN Hongxin(Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)
出处
《上海海洋大学学报》
CAS
CSCD
北大核心
2022年第4期873-882,共10页
Journal of Shanghai Ocean University
基金
上海市科学技术委员会项目(14320501900)。
关键词
枯草芽孢杆菌
添加剂量
絮团营养
菌群结构
Bacillus subtilis
additive amount
microbial community
biofloc nutrition
