摘要
目的:探讨壮药扶正方含药血清对肿瘤相关巨噬细胞(TAMs)与肺癌A549细胞共培养体系肺癌A549细胞增殖、迁移及侵袭等生物学行为影响及可能的作用机制。方法:采用氟波酯(PMA)联合白细胞介素-4、白细胞介素-13诱导人急性白血病单核细胞株(THP-1)建立TAMs体外模型,实时定量PCR及酶联免疫吸附试验法分析含药血清(低、中、高剂量组)及药物处理后24、48、72 h对TAMs表型调节;采用共培养体系,以肺癌A549细胞为研究对象,分别采用MTT法、实时细胞分析技术(RTCA)、Transwell法检测不同条件下A549细胞增殖、迁移及侵袭情况。结果:壮药扶正方含药血清能显著上调TMAs中M1型标志物白细胞介素-12、诱导型一氧化氮合酶表达水平,下调M2型标志物白细胞介素-10、CD206表达水平;与空白对照组比较,共培养组A549细胞增殖明显增加,细胞迁移及侵袭能力均明显升高,差异有统计学意义(P<0.05);与共培养组比较,壮药扶正方含药血清低、中、高剂量组A549细胞增殖、迁移和侵袭作用受抑制,且呈剂量依赖性(P<0.05)。结论:壮药扶正方含药血清在共培养体系中能够抑制肺癌A549细胞的增殖、迁移及侵袭作用,机制可能与其调节肿瘤微环境TAMs表型有关。
Objective:To investigate the effect of serum containing Zhuang medicine Fuzheng Prescription on proliferation,migration,invasion,and other biological behaviors of lung cancer A549 cells in the co-culture system composed of tumor-associated macrophages(TAMs)and lung cancer A549 cells and its possible mechanism.Methods:Human acute monocytic leukemic cell line(THP-1)was induced by phorbol-12-myristate-13-acetate(PMA)combined with interleukin(IL)-4 and IL-13,to establish the in vitro model of TAMs.Real-time quantitative polymerase chain reaction(RT-PCR)and enzyme-linked immunosorbent assay(ELISA)were used to analyze the phenotypic regulation of TAMs by medicine-containing serum(low,medium,and high-dose groups)and 24,48,and 72 h after administration.By using the supernatant co-culture system and taking lung cancer A549 cells as the research object,methyl thiazolyl tetrazolium(MTT)assay,real-time cell analysis(RTCA)technology,and Transwell assay were used to determine the proliferation,migration,and invasion of A549 cells under different conditions.Results:The serum containing Zhuang medicine Fuzheng Prescription significantly up-regulated the expression of M1-type markers IL-12 and inducible nitric oxide synthase(iNOS),and down-regulated the expression of M2-type markers IL-10 and CD206.As compared with the blank group,the proliferation of A549 cells in the co-culture group was increased,and the migration and invasion of A549 cells were improved(P<0.05).As compared with the co-culture group,the proliferation,migration,and invasion of A549 cells in the low,medium,and high-dose medicine-containing serum groups were inhibited in a dose-dependent manner(P<0.05).Conclusion:The serum containing Zhuang medicine Fuzheng Prescription can inhibit the proliferation,migration,and invasion of lung cancer A549 cells in the co-culture system,and the mechanism may be related to the regulation of the phenotype of TAMs in the tumor microenvironment.
作者
张顺荣
莫娟梅
甘芷川
李琼谦
李婵娟
郑盼
梁潇
唐伟智
唐璜
苏运
文海迪
黄振飞
ZHANG Shunrong;MO Juanmei;GAN Zhichuan;LI Qiongqian;LI Chanjuan;ZHENG Pan;LIANG Xiao;TANG Weizhi;TANG Huang;SU Yun;WEN Haidi;HUANG Zhenfei(Department of Oncology,Guangxi International Zhuang Medicine Hospital,Nanning 530201,China;Guangxi University of Chinese Medicine,Nanning 530200,China)
出处
《世界中医药》
CAS
2022年第14期2002-2007,共6页
World Chinese Medicine
基金
广西壮族自治区中医药管理局自筹课题(GZZC2020133)
广西中医药大学2019-2021年广西一流学科建设开放课题(2019XK065)
广西国际壮医医院资助课题(2019005)。
关键词
壮药扶正方
含药血清
肿瘤相关巨噬细胞
肺癌A549细胞
共培养体系
增殖
迁移
侵袭
Zhuang medicine Fuzheng Prescription
Medicine-containing serum
Tumor-associated macrophages
Lung cancer A549 cells
Co-culture system
Proliferation
Migration
Invasion
