摘要
【目的】研究不同富硒芽孢杆菌对富硒赤红壤硒形态、价态转化及菜心吸收累积硒的影响,为天然富硒蔬菜生产提供理论参考。【方法】以富硒赤红壤作为供试土壤、菜心作为供试作物,以2株富硒芽孢杆菌Bacillus cereus YLB1-6和Bacillus thuringiensis TXB2-5作为供试菌株,采用盆栽试验,以无菌水为对照(CK),接种不同菌液处理,收获后采样测定土壤硒形态和价态、pH、植株硒含量、植株生物量及硒累积量。【结果】接种富硒芽孢杆菌能促进残渣态硒(RES-Se)向不同硒形态的转化,但不同芽孢杆菌处理对土壤硒的形态转化存在一定的差异。YLB1-6和TXB2-5处理的土壤有效硒(AVA-Se)均显著增加(P<0.05,下同),主要由可溶态Se^(6+)(SOL-Se^(6+))和可交换态及碳酸盐结合态Se^(2-)(EX-Se^(2-))含量显著增加引起的,且2种价态硒含量YLB1-6处理均高于TXB2-5处理。YLB1-6处理能显著提高土壤pH。不同的富硒芽孢杆菌处理对菜心硒吸收累积效果不同,YLB1-6和TXB2-5处理均能显著提高菜心地上部硒含量;YLB1-6处理的菜心地下部硒含量与CK相当,而TXB2-5处理菜心地下部硒含量较CK显著增加;YLB1-6处理对于促进硒往菜心地上部转移的能力更强。接种富硒芽孢杆菌对菜心生物量无显著影响(P>0.05),但由于接种富硒芽孢杆菌能显著提高菜心地上部硒含量,菜心地上部硒累积量也相应显著增加。【结论】富硒芽孢杆菌能提高土壤有效硒含量,促进菜心地上部对土壤硒的吸收累积。其中YLB1-6对于促进植株地上部硒吸收效果更好,具有较好的田间应用潜力。
【Objective】To investigate the effects of different selenium(Se)-rich bacillus on Se speciation,valence transformation in Se-rich lateritic soil and Se absorption and accumulation in Chinese flowering cabbage,so as to provide reference for natural Se-rich vegetable production.【Method】Se-rich lateritic soil was used as tested soil and the Chinese flowering cabbage test crop.Two Se-rich Bacillus,Bacillus cereus YLB1-6 and Bacillus thuringiensis TXB2-5 were used as the test strains treated with different bacteria solutions and sterile water was taken as control(CK).Pot experiment method was adopted.Se speciation,valences and pH of the soil samples collected after harvest,biomass of plants,Se content and Se accumulation in plant were measured.【Result】Se-rich bacillus inoculation treatments promoted the transformation of residual Se(RES-Se)to different Se speciation,but there was some difference in Se speciation transforma-tions in the soil by different bacillus.Available Se(AVA-Se)in soil treated with YLB1-6 and TXB2-5 was significantly increased(P<0.05,the same below),and it was mainly caused by the significant increase of soluble Se^(6+)(SOL-Se^(6+))and exchangeable and carbonate-bound Se^(2-)(EX-Se^(2-))contents.And contents of these two Se valences were significantly hig her in YLB1-6 treatment than those in TXB2-5 treatment.YLB1-6 treatment significantly increased soil pH.Different Se rich bacillus treatments had different effects on the Se absorption and accumulation of Chinese flowering cabbage.Both YLB1-6 and TXB2-5 treatments could significantly increase Se content in above-ground parts of Chinese flowering cab-bage;Se content in below-ground parts of Chinese flowering cabbage in YLB1-6 treatment was similar to that of control,while TXB2-5 treatment significantly increased Se content in below-ground part of Chinese flowering cabbage.YLB1-6 treatment had a stronger ability to promote Se transfer to the above-ground parts of Chinese flowering cabbage.Se-rich ba-cillus inoculation had no obvious effe
作者
廖青
邢颖
梁潘霞
潘丽萍
黄太庆
陈锦平
江泽普
刘永贤
LIAO Qing;XINGYing;LIANG Pan-xia;PAN Li-ping;HUANG Tai-qing;CHEN Jin-ping;JIANG Ze-pu;LIU Yong-xian(Agricultural Resource and Environmental Research Institute,Guangxi Academy of Agricultural Sciences/Selenium Enriched Agriculture Research Center of Guangxi,Nanning,Guangxi 530007,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2022年第6期1666-1673,共8页
Journal of Southern Agriculture
基金
国家自然科学基金项目(32160762)
广西重点研发计划项目(桂科AB19245005)
广西青年基金项目(2018GXNSFBA281161)
广西农业科学院基本科研业务专项项目(桂农科2021JM08)。
关键词
富硒芽孢杆菌
硒形态
硒价态
硒含量
富硒赤红壤
菜心
Se-rich bacillus
Se speciation
Se valences
Se content
Se-rich lateritic red soil
Chinese flowering cabbage
