摘要
目的探究小鼠心肌细胞的缺血/再灌注(I/R)模型中缺氧诱导因子-1α(HIF-1α)对白细胞介素-2(IL-2)的表达调控作用和作用机制。方法将60只小鼠随机分为对照组、I/R组、I/R+HIF-1α组和I/R+siHIF-1α组四组(每组各15例)。采用经典结扎法构建I/R模型,尾静脉注射HIF-1α过表达或siHIF-1α质粒(2 mg/kg)。注射等量阴性对照质粒作为对照。2周后检测各组小鼠的HIF-1α蛋白、心肌损伤指标、心肌梗死面积、细胞凋亡、IL-2、VEGF mRNA和蛋白的水平。结果四组小鼠的上述指标比较,差异均有统计学意义(P<0.05)。I/R组的HIF-1α蛋白、肌酸激酶同工酶(CK-MB)、肌钙蛋白T(cTnT)、心肌梗死面积、凋亡指数、IL-2和VEGF mRNA和蛋白的水平,显著高于对照组(P<0.05)。I/R+HIF-1α组的HIF-1α蛋白、IL-2、VEGF mRNA和蛋白的水平显著高于I/R组,CK-MB、cTnT、梗死百分比、凋亡指数显著低于I/R组(P<0.05)。I/R+siHIF-1α组的HIF-1α蛋白、IL-2、VEGF mRNA和蛋白的水平显著低于I/R组,CK-MB、cTnT、心肌梗死面积、凋亡指数显著高于I/R组(P<0.05)。结论小鼠心肌细胞的I/R模型中HIF-1α可通过促进IL-2的表达缓解心肌组织损伤。
Objective To investigate the regulation effect of hypoxia-inducible factor 1α(HIF-1α)on interleukin-2(IL-2)expression in mouse model of myocardial ischemia reperfusion(I/R),and study the effective mechanism.Methods All mice(n=60)were divided into control group,I/R group,I/R+HIF-1αgroup and I/R+siHIF-1αgroup(each n=15).The mouse model of myocardial I/R was established by applied classic ligation.The overexpressed HIF-1αor siHIF-1αplasmids(2 mg/kg)were injected into mouse caudal vein.The injection of negative control plasmid in equal amount was taken as control.The level of HIF-1αprotein,indexes of myocardial injury,infarction area,apoptosis,and level of interleukin-2(IL-2)and mRNA and protein levels of vascular endothelial growth factor(VEGF mRNA,VEGF protein)were detected in all groups after 2 weeks.Results The differences in all above indexes had statistical significance among 4 groups(P<0.05).The levels of HIF-1αprotein,creatine kinase-MB isoenzyme(CK-MB)and cardiac troponin I(cTnI),infarction area,apoptosis index,and levels of IL-2,VEGF mRNA and VEGF protein were significantly higher in I/R group than those in control group(P<0.05).The levels of HIF-1αprotein,IL-2,VEGF mRNA and VEGF protein were significantly higher,and CK-MB,cTnT,infarction percentage and apoptosis index were significantly lower in I/R+HIF-1αgroup than those in I/R group(P<0.05).The levels of HIF-1αprotein,IL-2,VEGF mRNA and VEGF protein were significantly lower,and CK-MB,cTnT,infarction area and apoptosis index were significantly higher I/R+siHIF-1αgroup than those in I/R group(P<0.05).Conclusion HIF-1αcan relieve myocardial injury through improving IL-2 expression in mouse model of myocardial I/R.
作者
黄仲略
杨威
黄菲菲
高翔
Huang Zhonglue;Yang Wei;Huang Feifei;Gao Xiang(Xiehe Jiangbei Hospital,Huazhong University of Science and Technology,Wuhan 430100,China;不详)
出处
《中国循证心血管医学杂志》
2022年第7期816-819,共4页
Chinese Journal of Evidence-Based Cardiovascular Medicine
基金
湖北省教育厅科学技术研究计划指导性项目(B2018001)。
