摘要
建立了基于铜纳米簇(CuNCs)的免标记荧光生物传感器快速检测链霉素的方法。利用链霉素核酸适配体和双链茎设计并合成了发夹结构DNA(hpDNA)模板,富含AT的双链茎部分可以合成CuNCs。在CuNCs荧光传感体系中加入链霉素后,链霉素与核酸适配体结合,使hpDNA双链茎打开,CuNCs荧光强度明显下降。在优化条件下,该方法检测链霉素的线性范围为0.1~20 mg/L,线性回归方程为y=240.17-10.31x,线性回归系数r^(2)为0.9954,检出限为4.36μg/L。该方法对牛奶样品的加标回收率为98.6%~104.8%。方法无需复杂的DNA序列设计、荧光标记,实验操作简单易行,适用于链霉素的快速检测。
A rapid and label-free fluorescent sensing detection method has been developed for streptomycin by using copper nanoclusters biosensor.The hairpin DNA(hpDNA)template contains a streptomycin aptamer loop and a AT-rich double strand stem,and the AT-rich sequences are the template for the formation of fluorescent CuNCs.When streptomycin is present in the hpDNA-CuNCs solution,the fluorescence intensity decreased effectively,due to the structure switching of the hpDNA.Under optimized conditions,the method shows a good linear relationship in the streptomycin concentration range of 0.1~20 mg/L,and the correlation coefficients(r^(2))is 0.9954.The detection limit of this method is 4.36μg/L.Furthermore,the method is successfully applied to the detection of streptomycin in milk samples with recoveries ranging from 98.6%to 104.8%.The method does not require complicated DNA sequence design and fluorescent labeling,the experimental operation is simple,and is suitable for the rapid detection of streptomycin.
作者
杨丽霞
易姿
黄小贝
汪胜
Yang Lixia;Yi Zi;Huang Xiaobei;Wang Sheng(Changsha Institute for Food and Drug Control;Food Safety Production Engineering Research Center of Hunan Province;National Alcohol Products Quality Supervision and Inspection Center,Changsha,410036)
出处
《化学通报》
CAS
CSCD
北大核心
2022年第8期971-975,897,共6页
Chemistry
基金
湖南省自然科学基金科药联合基金项目(2021JJ80077)
国家市场监督管理总局科技计划项目(2020MK074)
湖南省市场监督管理局科技计划项目(2020KJJH39)资助。
