摘要
目的研究黄芪对细胞毒素相关蛋白A(cagA)诱导大鼠的系膜细胞外基质分泌的影响。方法将大鼠系膜细胞进行体外培养,并随机分为四组,分别为空白对照组、IL-1β阳性对照组(10 ng/ml IL-1β)和CagA实验组(4μg/ml CagA)及CagA+黄芪干预组(1 mg/ml的黄芪注射液+4μg/ml CagA),各组予以对应处理后刺激培养72 h,检测细胞培养上清液Ⅰ型胶原(CollagenⅠ)、Ⅲ型胶原(CollagenⅢ型)蛋白水平及其mRNA表达。结果IL-1β阳性对照组、CagA实验组CollagenⅠ、CollagenⅢ蛋白浓度及mRNA表达水平高于空白对照组,CagA+黄芪干预组CollagenⅠ、CollagenⅢ蛋白浓度及mRNA表达水平低于CagA实验组,差异有统计学意义(P<0.05)。结论黄芪或通过减少Ⅰ、Ⅲ型胶原蛋白的分泌,抑制CagA诱导的大鼠系膜细胞外基质的异常分泌,可为IgA肾病的治疗提供思路。
Objective To investigate the effects of Astragalus Membranaceus on CagA-induced extracellular matrix secretion of rat mesangial cells.Methods Rat mesangial cells were cultured in vitro and randomly divided into four groups:blank control group,IL-1βpositive control group(10 ng/ml IL-1β),CagA experimental group(4μg/ml CagA)and CagA+Astragalus Membranaceus intervention group(1 mg/ml Astragalus Membranaceus injection+4μg/ml CagA).Each group was stimulated for 72 h after corresponding treatment.The protein level and mRNA expression of typeⅠcollagen(CollagenⅠ)and typeⅢcollagen(CollagenⅢ)in cell culture supernatant were detected.Results The protein concentration and mRNA expression of CollagenⅠand CollagenⅢin IL-1βpositive control group and CagA experimental group were higher than those in blank control group,and the protein concentration and mRNA expression of CollagenⅠand CollagenⅢin CagA+Astragalus Membranaceus intervention group were lower than those in CagA experimental group,the difference was statistically significant(P<0.05).Conclusion Astragalus Membranaceus can inhibit CagA-induced abnormal secretion of extracellular matrix in rat mesangial cells by reducing the secretion of typeⅠand typeⅢcollagen,which can provide ideas for the treatment of IgA nephropathy.
作者
陈越
彭莉
CHEN Yue;PENG Li(Department of Nephrology,Zigong First People's Hospital,Zigong 643000,Sichuan,China;Department of Nephrology,Chengdu Seventh People's Hospital,Chengdu 610000,Sichuan,China)
出处
《医学信息》
2022年第15期104-106,124,共4页
Journal of Medical Information
关键词
IGA肾病
黄芪
系膜细胞
细胞毒素相关蛋白A
IgA nephropathy
Astragalus Membranaceus
Mesangial cells
Cytotoxin-associated protein A
