摘要
目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)、血管内皮生长因子受体1(vascular endothelial growth factor receptor 1,VEGFR1)通路相关基因的多态性与子痫前期发病风险的相关性。方法选择178例子痫前期孕妇和100例正常孕晚期孕妇分别纳入病例组及对照组,采用PCR-限制性片段长度多态性方法检测VEGF基因rs3025039、rs2010963以及VEGFR1基因rs3812867、rs55875014、rs722503位点的多态性,同时测定孕妇血清的VEGF及sVEGFR1水平,分析结果与子痫前期发生的相关性。结果病例组收缩压、舒张压、sVEGFR1均显著高于对照组,VEGF显著低于对照组(P<0.05)。所有位点的基因型分布均满足Hardy-Weinberg平衡(P>0.05)。病例组VEGF rs3025039位点的T等位基因频率高于对照组,在显性、共显性模型下均有统计学意义(P<0.05)。与CC型相比,CT和TT型患病风险较高,其收缩压、舒张压、sVEGFR1显著偏高,VEGF水平显著偏低(P<0.05)。两组其余位点的等位基因频率差异均无统计学意义(P>0.05)。结论VEGF基因rs3025039位点与子痫前期的发生相关,该位点的变异可能影响VEGF的活性,介导子痫前期的发生及发展。
Objective To assess the association of single nucleotide polymorphisms(SNPs)of vascular endothelial growth factor(VEGF)and vascular endothelial growth factor receptor 1(VEGFR1)pathways-related genes and the risk of pre-eclampsia.Methods In total 178 pregnant women with pre-eclampsia(case group)and 100 healthy pregnant women(control group)during the third trimester were enrolled.The SNPs of VEGF rs3025039,rs2010963 and VEGFR1 rs3812867,rs55875014 and rs722503 loci were determined by PCR-restriction fragment length polymorphism(PCR-RFLP)assay.The levels of serum VEGF and sVEGFR1 were also determined.And their association with pre-eclampsia was analyzed.Results The systolic blood pressure,diastolic blood pressure and sVEGFR1 of the case group were significantly higher than those of the control group,while the VEGF level was significantly lower than that in the control group(P<0.05).Allelic frequencies of the VEGF(rs3025039,rs2010963)and VEGFR1(rs3812867,rs55875014,rs722503)have fit the Hardy-Weinberg equilibrium(P>0.05).The frequency of T allele of VEGF at rs3025039 locus in the case group was higher than that in the control group(P<0.05).There were significant differences in VEGF at rs3025039 locus under dominant and co-dominant models in case group(P<0.05).Compared with those with CC,the risk was higher in patients with CT or TT genotypes(P<0.05).The systolic and diastolic blood pressure and sVEGFR1 in pre-eclampsia pregnant women with CT or TT genotypes were significantly higher than those with the CC genotype,while their VEGF level was significantly lower(P<0.05).No significant difference was found in allelic frequencies of other four loci between the two groups(P>0.05).Conclusion Polymorphisms of rs3025039 locus of VEGF gene is associated with the occurrence of pre-eclampsia.The variant at this locus may affect the activity of VEGF and influence the development of pre-eclampsia.
作者
李力
郭祥翠
陈贝贝
郜智慧
刘娟
王倩青
Li Li;Guo Xiangcui;Chen Beibei;Gao Zhihui;Liu Juan;Wang Qianqing(Department of Gynecology and Oncology,Xinxiang Central Hospital,Xinxiang,Henan 453000,China;Department of Gynecology,the Third Affiliated Hospital of Guangzhou Medical University,Guangzhou,Guangdong 510150,China)
出处
《中华医学遗传学杂志》
CAS
CSCD
2022年第8期893-897,共5页
Chinese Journal of Medical Genetics
基金
国家自然科学基金 (81671440)。
关键词
子痫前期
血管内皮生长因子
血管内皮生长因子受体1
基因多态性
Pre-eclampsia
Vascular endothelial growth factor
Vascular endothelial growth factor receptor 1
Genetic polymorphism
