摘要
为筛选耐涝丝瓜砧木,探讨丝瓜耐涝机制,采用双因素完全随机设计研究了淹水胁迫对3个丝瓜品种根系呼吸生理和通气组织的影响。结果表明,淹水胁迫4 d,3个丝瓜品种的根系干质量均显著低于对照;淹水胁迫4~16 d,3个丝瓜品种主根根系活力均显著低于对照;淹水胁迫8~16 d,3个丝瓜品种不定根的根系活力均显著高于对照。淹水胁迫2~16 d,3个丝瓜品种主根的乳酸脱氢酶(LDH)、丙酮酸脱羧酶(PDC)活性均显著高于对照;淹水胁迫2~16 d开始,除绿冠丝瓜与对照差异不显著外,其他2个品种主根中乙醇脱氢酶(ADH)活性均显著高于对照。3个丝瓜品种不定根中均形成了通气组织。在淹水胁迫下,早佳丝瓜根系干质量最大,达到236.7 mg,分别比荆李和绿冠高出43.98%和100.59%;最早形成不定根,通气组织最发达;不定根的根系活力最强,达到7.73 mg·g^(-1)·h^(-1),分别比荆李和绿冠高出22.40%和64.44%;不定根的PDC活性和ADH活性应急反应最快,淹水胁迫2 d就显著高于对照,分别比荆李和绿冠早2 d和6 d。因此,3个丝瓜品种均耐淹,早佳丝瓜最耐淹水,是较理想的耐涝丝瓜砧木。
In order to screen waterlogging-resistant luffa rootstocks and explore the mechanism of waterlogging-resistant luffa,the effects of waterlogging stress on root respiratory physiology and aerated tissue of three luffa varieties were studied by two-factor completely random design.The results showed that the root dry weight of the three varieties of luffa was significantly lower than that of the control after 4 days of waterlogging stress.After 4 to 16 days of waterlogging stress,the root activity of the main root of the three varieties of luffa was significantly lower than that of the control.Root activity of adventitious roots of the three luffa cultivars was significantly higher than that of the control during 8 to 16 days of waterlogging stress.The activity of lactate dehydrogenase(LDH)and pyruvate decarboxylase(PDC)of the main roots of the three varieties of luffa were significantly higher than those of the control during 2 to 16 days of waterlogging stress.The activity of ethanol dehydrogenase(ADH)in the main root of luffa was significantly higher than that of the control except that there was no significant difference between Lüguan luffa and the control from 2 to 16 days after waterlogging stress.Aerenchyma was formed in adventitious roots of three luffa varieties.Among the three varieties,the root dry weight of Zaojia luffa was the highest,reaching 236.7 mg,43.98%and 100.59%higher than that of Jingli and Lüguan,respectively.Adventitious roots were first formed and aerenchyma was most developed.The root activity of adventitious roots were 7.73 mg·g^(-1)·h^(-1),22.40%and 64.44%higher than that of Jingli and Lüguan,respectively.The PDC activity and ADH activity of adventitious roots were the fastest in emergency response.The PDC activity and ADH activity of adventitious roots were significantly higher than those of the control at 2 d under waterlogging stress,and were 2 d and 6 d earlier than those of Jingli and Lüguan,respectively.Therefore,the three luffa varieties are all waterlogging resistant,among
作者
朱进
李文静
徐兰婷
ZHU Jin;LIWenjing;XU Lanting(College of Horticulture and Gardening,Yangtze University,Jingzhou 434025,Hubei,China;Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement,Wuhan 430070,Hubei,China)
出处
《中国瓜菜》
CAS
北大核心
2022年第8期62-69,共8页
China Cucurbits And Vegetables
基金
湖北省重点研发计划项目(2020BBA037)
长江大学湿地生态与农业利用教育部工程中心开放基金(KF201803)
武汉市农业科学院开放性课题(KFXKT201801)
长江大学大学生创新创业训练计划项目(S202110489087/Yz2020368)。
关键词
丝瓜
淹水胁迫
无氧呼吸
通气组织
Luffa
Waterlogging stress
Anaerobic respiration
Aerenchyma
