摘要
目的观察中等强度适量运动干预对自发性高血压大鼠(SHR)左心室重塑(如心肌细胞肥大、凋亡和增殖)的影响及可能机制。方法采用随机数字表法将30只4月龄雌性SHR大鼠分为安静组和运动组,每组15只,另选取15只Wistar Kyoto大鼠纳入对照组。运动组大鼠给予12周中等强度跑台运动,每天运动60 min,每周运动5 d,共持续干预12周,同期安静组及对照组大鼠则置于鼠笼内安静饲养。经12周干预后,采用无创血压测试仪测量各组大鼠尾动脉血压,然后处死大鼠取心脏进行形态计量学测定,分离心肌细胞,并采用DAPI染色法测量其长度、宽度及面积,采用TUNEL法检测心肌细胞凋亡情况,采用免疫荧光染色法检测心肌细胞增殖率,采用流式细胞术检测心脏祖细胞数量,采用Western blot法检测心肌钙调神经磷酸酶Aβ亚基(CNAβ)和磷酸化Akt(p-Akt)蛋白表达量。结果与对照组比较,安静组大鼠心脏重量、心脏质量指数(HMI)、收缩压、舒张压、左心室壁(前壁、后壁和间隔壁)心肌厚度、心肌细胞形态参数(长度、宽度和面积)、心肌细胞凋亡率、增殖率、心脏祖细胞数量以及CNAβ蛋白表达量均显著增加(P<0.05);与安静组比较,运动组大鼠心脏重量、HMI、左心室壁(前壁、后壁和间隔壁)心肌厚度、心肌细胞形态参数(长度、宽度和面积)、心肌细胞增殖率、心脏祖细胞数量以及p-Akt蛋白表达量均显著增加(P<0.05),收缩压、舒张压、细胞凋亡率以及CNAβ蛋白表达量则显著降低(P<0.05)。结论中等强度适量运动干预能诱导SHR大鼠心脏生理性肥大、减轻细胞凋亡、增加心脏祖细胞数量并促进细胞增殖,进而抑制心脏重塑。
Objective To observe any effect of moderate-intensity exercise on left ventricular remodeling(such as cardiomyocyte hypertrophy,apoptosis and proliferation)in spontaneously-hypertensive rats(SHRs)and explore the possible mechanisms.Methods Thirty 4-month-old female SHRs were randomly divided into a sedentary group(n=15)and an exercise group(n=15).Fifteen Wistar Kyoto rats served as the control group.The exercise group underwent daily 60-min moderate-intensity treadmill exercise 5 days per week for 12 weeks,while the sedentary and control groups were raised quietly in cages for the same period.After the 12-week intervention,the caudal artery blood pressure was measured using a non-invasive blood pressure monitor.The rats were then sacrificed and their hearts were sampled for morphometric measurement.Cardiomyocytes were isolated and underwent DAPI staining to measure their length,width and area.Apoptosis cardiomyocytes was detected by using terminal-deoxynucleoitidyl transferase mediated nick end labeling and their proliferation was assessed using immunofluorescent staining.The number of cardiac progenitor cells was detected by flow cytometry,while the expression of the cardiac calcineurin Aβsubunit(CNAβ)and phosphorylated Akt(p-Akt)protein were measured using western blotting.Results Compared with the control group,a significant increase was observed in the heart weight,heart mass index(HMI),systolic blood pressure,diastolic blood pressure,myocardial thickness of the left ventricular wall(anterior wall,posterior wall and septal wall),cardiomyocyte morphology(length,width and area),cardiomyocyte apoptosis rate,proliferation rate,number of cardiac progenitor cells and protein expression of CNAβin the sedentary group.Compared with the sedentary group,the average heart weight,HMI,myocardial thickness of the left ventricular wall(anterior wall,posterior wall and septal wall),cardiomyocyte morphology(length,width and area),cardiomyocyte proliferation rate,number of cardiac progenitor cells and p-Akt protein expressio
作者
马琳
刘晓哲
王天添
Ma Lin;Liu Xiaozhe;Wang Tiantian(Physical Education Department,Luoyang Institute of Science and Technology,Luoyang 471023,China;The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450002,China;Health Service,Logistics University of the People′s Armed Police,Tianjin 300309,China)
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2022年第7期589-594,共6页
Chinese Journal of Physical Medicine and Rehabilitation
基金
河南省科技攻关项目(222102320127)。
关键词
运动
自发性高血压大鼠
左心室重塑
心肌肥厚
细胞凋亡
Exercise
Spontaneous hypertension
Left ventricle
Remodeling
Myocardial hypertrophy
Apoptosis
